• Article |

    Cryo-electron microscopy studies show that dynamic coordination of Na+ in the ion channel of Dispatched homologue 1 and the transmembrane Na+ gradient have key roles in exporting lipid-modified Hedgehog protein signal.

    • Qianqian Wang
    • , Daniel E. Asarnow
    •  & Philip A. Beachy
  • Article |

    Experiments using endogenous and biomimetic condensates in cells show that nucleation in cells resembles the physical process in inanimate materials, but is tuned by biomolecular features.

    • Shunsuke F. Shimobayashi
    • , Pierre Ronceray
    •  & Clifford P. Brangwynne
  • Article |

    Phase separation properties are a major determinant of UTX activity in chromatin regulation in tumour suppression, and are dependent on a core intrinsically disordered region of the protein.

    • Bi Shi
    • , Wei Li
    •  & Hao Jiang
  • Article |

    Structural, functional and localization studies reveal that Geobacter sulfurreducens pili cannot behave as microbial nanowires, instead functioning in a similar way to secretion pseudopili to export cytochrome nanowires that are essential for extracellular electron transfer.

    • Yangqi Gu
    • , Vishok Srikanth
    •  & Nikhil S. Malvankar
  • Article
    | Open Access

    AlphaFold predicts protein structures with an accuracy competitive with experimental structures in the majority of cases using a novel deep learning architecture.

    • John Jumper
    • , Richard Evans
    •  & Demis Hassabis
  • Article |

    A structure of the sperm-specific CatSper complex features a number of additional components; together, these components and the CatSper complex are termed the CatSpermasome.

    • Shiyi Lin
    • , Meng Ke
    •  & Jianping Wu
  • Article |

    Cryptochrome 4 from the night-migratory European robin displays magnetically sensitive photochemistry in vitro, in which four successive flavin–tryptophan radical pairs generate magnetic-field effects and stabilize potential signalling states.

    • Jingjing Xu
    • , Lauren E. Jarocha
    •  & P. J. Hore
  • Article |

    A localization algorithm is applied to datasets obtained with conventional and high-speed atomic force microscopy to increase image resolution beyond the limits set by the radius of the tip used.

    • George R. Heath
    • , Ekaterina Kots
    •  & Simon Scheuring
  • Perspective |

    Inanimate matter is beginning to show some signs of basic intelligence—the ability to sense, actuate and use memory, as controlled by an internal communication network in functional materials.

    • C. Kaspar
    • , B. J. Ravoo
    •  & W. H. P. Pernice
  • Article |

    Cryo-electron microscopy structures of PCFT in a substrate-free state and bound to the antifolate drug pemetrexed provide insights into how this protein recognizes folates and mediates their transport into cells.

    • Joanne L. Parker
    • , Justin C. Deme
    •  & Simon Newstead
  • Article
    | Open Access

    Cryo-electron microscopy structure of heterodimeric GABAB receptor in complex with Gi1 protein reveals that the mode of G-protein binding in this class-C G-protein-coupled receptor differs from that of other classes.

    • Cangsong Shen
    • , Chunyou Mao
    •  & Jianfeng Liu
  • Article |

    Structures of the human sodium-dependent citrate transporter NaCT in complexes with citrate or a small-molecule inhibitor reveal how the inhibitor—which binds to the same site as citrate—arrests the transport cycle of NaCT.

    • David B. Sauer
    • , Jinmei Song
    •  & Da-Neng Wang
  • Article |

    Mechanical load-sharing enables the long-range cooperative uptake of apoptotic cells by multiple epithelial cells; and clearance of these apoptotic cells facilitates error correction, which is necessary for developmental robustness and survival of the embryo.

    • Esteban Hoijman
    • , Hanna-Maria Häkkinen
    •  & Verena Ruprecht
  • Article |

    Multiplexed imaging of 3,660 chromosomal loci in individual mouse embryonic stem cells by DNA seqFISH+ with immunofluorescence of 17 chromatin marks and subnuclear structures reveals invariant organization of loci within individual cells, and heterogeneous and long-lived distinct combinatorial chromatin states in cellular subpopulations.

    • Yodai Takei
    • , Jina Yun
    •  & Long Cai
  • Article |

    A theoretical model, in vitro reconstitution and in vivo experimentation show that competition between droplet surface tension and membrane sheet instability dictates the form and function of autophagosomal membranes.

    • Jaime Agudo-Canalejo
    • , Sebastian W. Schultz
    •  & Roland L. Knorr
  • Article |

    A high-throughput, chromosome-wide analysis of DNA looping reveals its contribution to the organization of chromatin, and provides insight into how nucleosomes are deposited and organised de novo.

    • Aakash Basu
    • , Dmitriy G. Bobrovnikov
    •  & Taekjip Ha
  • Article |

    Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.

    • Robert Dods
    • , Petra Båth
    •  & Richard Neutze
  • Article |

    High-resolution cryo-electron microscopy structure of smooth muscle myosin II in the inhibited state enables increased understanding of the functions of the head and tail regions in regulation of myosin activity and the pathological mechanisms of disease mutations.

    • Shixin Yang
    • , Prince Tiwari
    •  & Roger Craig
  • Article |

    Cryo-electron microscopy structures of GABAA receptors bound to intravenous anaesthetics and benzodiazepines reveal both common and distinct transmembrane binding sites, and show that the mechanisms of action of anaesthetics partially overlap with those of benzodiazepines.

    • Jeong Joo Kim
    • , Anant Gharpure
    •  & Ryan E. Hibbs
  • Article |

    The surfactant-like protein Ki-67 mediates the clustering of chromosomes during mitotic exit, which displaces large cytoplasmic molecules from the future nuclear space and thus enables the separation of cytoplasmic and nuclear components before the nuclear envelope reforms.

    • Sara Cuylen-Haering
    • , Mina Petrovic
    •  & Daniel W. Gerlich
  • Article |

    The adaptability of the plant Arabidopsis thaliana to different temperatures is regulated by the ability of its ELF3 protein to undergo liquid–liquid phase separation, in a manner that is dependent on the protein’s prion-like domain.

    • Jae-Hoon Jung
    • , Antonio D. Barbosa
    •  & Philip A. Wigge
  • Article |

    An approach for the design of protein pores is demonstrated by the computational design and subsequent experimental expression of both an ion-selective and a large transmembrane pore.

    • Chunfu Xu
    • , Peilong Lu
    •  & David Baker
  • Article |

    The chromatin protein MeCP2 is a component of dynamic, liquid-like heterochromatin condensates, and the ability of MeCP2 to form condensates is disrupted by mutations in the MECP2 gene that occur in the neurodevelopmental disorder Rett syndrome.

    • Charles H. Li
    • , Eliot L. Coffey
    •  & Richard A. Young
  • Article |

    Single-molecule fluorescence resonance energy transfer and real-time confocal laser tracking with fluorescence correlation spectroscopy together characterize how individual lac repressor molecules bypass operator sites while exploring the DNA surface at microsecond timescales.

    • Emil Marklund
    • , Brad van Oosten
    •  & Sebastian Deindl