Stem cells

  • Article
    | Open Access

    Blastoids derived from naive PXGL-cultured human pluripotent stem cells in which Hippo, TGF-β and ERK pathways are inhibited closely recapitulate aspects of blastocyst development, form cells resembling blastocyst-stage cells and thus provide a model system for implantation and development studies.

    • Harunobu Kagawa
    • , Alok Javali
    •  & Nicolas Rivron
  • Article |

    The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization reveals parallels and differences in gastrulation in humans as compared with mouse and non-human primate models.

    • Richard C. V. Tyser
    • , Elmir Mahammadov
    •  & Shankar Srinivas
  • Article
    | Open Access

    RNA-sequencing analysis of the prenatal human brain at different stages of development shows that areal transcriptional signatures are dynamic and coexist with developmental and cell-type signatures.

    • Aparna Bhaduri
    • , Carmen Sandoval-Espinosa
    •  & Arnold R. Kriegstein
  • Article |

    A rare population of acinar cells expressing telomerase reverse transcriptase renew the acinar cell compartment during homeostasis, and are potential sources of premalignant cells in pancreatic carcinogenesis.

    • Patrick Neuhöfer
    • , Caitlin M. Roake
    •  & Steven E. Artandi
  • Article |

    An analysis of skeletal stem cells in mice reveals that bone ageing occurs at the level of local niches affecting skeletal and haematopoietic lineage output, which may influence systemic aspects of multi-organ physiological ageing.

    • Thomas H. Ambrosi
    • , Owen Marecic
    •  & Charles K. F. Chan
  • Article |

    Live imaging and single-cell transcriptomics of mouse hair follicles reveal their development from 2D concentric zones in the placode to 3D longitudinal compartments, one of which is a stem cell compartment.

    • Ritsuko Morita
    • , Noriko Sanzen
    •  & Hironobu Fujiwara
  • Article |

    Stress inhibits  hair growth in mice through the release of the stress hormone corticosterone from the adrenal glands, which inhibits the activation of hair follicle stem cells by suppressing the expression of a secreted factor, GAS6, from the dermal niche.

    • Sekyu Choi
    • , Bing Zhang
    •  & Ya-Chieh Hsu
  • Article |

    In a rat model of short bowel syndrome, transplantation of small intestinal organoids into the colon partially restores intestinal function and improves survival—a proof of principle that organoid transplantation might have therapeutic benefit.

    • Shinya Sugimoto
    • , Eiji Kobayashi
    •  & Toshiro Sato
  • Article |

    A combination of fluorescent antibodies is used to build visual maps of all myeloid cells in the bone marrow, providing new insight into how the bone marrow microenvironment regulates cell-fate decisions.

    • Jizhou Zhang
    • , Qingqing Wu
    •  & Daniel Lucas
  • Article |

    Specific macrophage populations provide a transient niche that activates muscle stem cells after muscle injury and supply proliferation-inducing cues that govern the repair process mediated by these cells in both zebrafish and mouse injury models.

    • Dhanushika Ratnayake
    • , Phong D. Nguyen
    •  & Peter D. Currie
  • Article |

    Primed pluripotent stem cells from distant species compete with each other, and inactivation of NF-κB signalling in normally outcompeted human cells improves their survival and chimerism in mouse embryos.

    • Canbin Zheng
    • , Yingying Hu
    •  & Jun Wu
  • Article |

    The rate of scale regeneration in zebrafish is controlled by the frequency of rhythmic travelling waves of Erk activity, which are broadcast from a central source to induce ring-like patterns of osteoblast tissue growth.

    • Alessandro De Simone
    • , Maya N. Evanitsky
    •  & Stefano Di Talia
  • Article |

    Expression of three Yamanaka transcription factors in mouse retinal ganglion cells restores youthful DNA methylation patterns, promotes axon regeneration after injury, and reverses vision loss in a mouse model of glaucoma and in aged mice, suggesting that mammalian tissues retain a record of youthful epigenetic information that can be accessed to improve tissue function.

    • Yuancheng Lu
    • , Benedikt Brommer
    •  & David A. Sinclair
  • Article |

    Depletion of TRF2—an essential mediator of telomere protection in most mammalian cells—in mouse embryonic stem cells activates a compensatory transcriptional program that renders TRF2 dispensable for their survival and proliferation.

    • Marta Markiewicz-Potoczny
    • , Anastasia Lobanova
    •  & Eros Lazzerini Denchi
  • Article |

    Analysis of 97,691 high-coverage human blood DNA-derived whole-genome sequences enabled simultaneous identification of germline and somatic mutations that predispose individuals to clonal expansion of haematopoietic stem cells, indicating that both inherited and acquired mutations are linked to age-related cancers and coronary heart disease.

    • Alexander G. Bick
    • , Joshua S. Weinstock
    •  & Pradeep Natarajan
  • Article |

    Single-cell analysis of blood vessels in the alveolus, the site of chronic disease and virus-induced lung injury, reveals two intermingled endothelial cell types with specialized gas exchange and stem cell functions.

    • Astrid Gillich
    • , Fan Zhang
    •  & Ross J. Metzger
  • Article |

    An organoid-based screening platform maps the genetic interactions underlying intestinal development and regeneration, showing that retinoic acid metabolism maintains the balance between regeneration and homeostasis, and that an antagonist of the retinoid X receptor promotes regeneration in vivo.

    • Ilya Lukonin
    • , Denise Serra
    •  & Prisca Liberali
  • Article |

    Miniature gut tubes grown in vitro from mouse intestinal stem cells are perfusable, can be colonized with microorganisms and exhibit a similar arrangement and diversity of specialized cell types to intestines in vivo.

    • Mikhail Nikolaev
    • , Olga Mitrofanova
    •  & Matthias P. Lutolf
  • Article |

    Metabolically-mature human islet-like organoids generated from induced pluripotent stem cells are able to recapitulate insulin-responsive pancreatic islet function and avoid immunologic cell death in diabetic mouse transplantation models.

    • Eiji Yoshihara
    • , Carolyn O’Connor
    •  & Ronald M. Evans
  • Article |

    Single-cell analysis in a mouse model of skin stretching shows that stretching causes a transient expansion bias in a population of epidermal stem cells, which is associated with chromatin remodelling and changes in transcriptional profiles.

    • Mariaceleste Aragona
    • , Alejandro Sifrim
    •  & Cédric Blanpain
  • Article |

    The DNA modification N6-methyladenine regulates gene expression during mouse trophoblast development by depositing at the boundaries of active chromatin and preventing its spread by antagonizing the chromatin organizer SATB1.

    • Zheng Li
    • , Shuai Zhao
    •  & Andrew Z. Xiao
  • Article |

    Single-molecule fluorescence in situ hybridization and live-cell imaging are used to study the contribution of transcriptional noise to stem cell heterogeneity, revealing that stochastic transcription dynamics are conducive to concomitant stem-cell maintenance and tissue homeostasis.

    • Justin C. Wheat
    • , Yehonatan Sella
    •  & Ulrich Steidl
  • Article |

    The authors show that metabolic activity leads to an increase in the intracellular pH of neuromesodermal precursors, and that this increase in pH, by allowing post-translational modification of β-catenin, is required for the activation of WNT signalling and mesodermal fate acquisition.

    • Masayuki Oginuma
    • , Yukiko Harima
    •  & Olivier Pourquié
  • Article |

    A set of five small molecules can induce the transformation of fibroblasts into rod photoreceptor-like cells, which can partially restore pupil reflex and visual function when transplanted into a rod degeneration mouse model.

    • Biraj Mahato
    • , Koray Dogan Kaya
    •  & Sai H. Chavala
  • Article |

    A system involving in vitro induction of presomitic mesoderm recapitulates oscillatory expression of core segmentation clock genes and travelling-wave-like gene expression, suggesting that this system can be used to study the human segmentation clock and provide insights into diseases associated with human axial skeletogenesis.

    • Mitsuhiro Matsuda
    • , Yoshihiro Yamanaka
    •  & Cantas Alev
  • Article |

    Single-cell RNA sequencing and spatial transcriptomics reveal that the somitogenesis clock is active in mouse gastruloids, which can be induced to generate somites with the correct rostral–caudal patterning.

    • Susanne C. van den Brink
    • , Anna Alemany
    •  & Alexander van Oudenaarden