Stem-cell differentiation

  • Article |

    A combination of fluorescent antibodies is used to build visual maps of all myeloid cells in the bone marrow, providing new insight into how the bone marrow microenvironment regulates cell-fate decisions.

    • Jizhou Zhang
    • , Qingqing Wu
    •  & Daniel Lucas
  • Article |

    Metabolically-mature human islet-like organoids generated from induced pluripotent stem cells are able to recapitulate insulin-responsive pancreatic islet function and avoid immunologic cell death in diabetic mouse transplantation models.

    • Eiji Yoshihara
    • , Carolyn O’Connor
    •  & Ronald M. Evans
  • Article |

    Single-molecule fluorescence in situ hybridization and live-cell imaging are used to study the contribution of transcriptional noise to stem cell heterogeneity, revealing that stochastic transcription dynamics are conducive to concomitant stem-cell maintenance and tissue homeostasis.

    • Justin C. Wheat
    • , Yehonatan Sella
    •  & Ulrich Steidl
  • Article |

    Human presomitic mesoderm cells derived in vitro demonstrate oscillations of the segmentation clock, thus providing a window into an otherwise inaccessible stage of human development.

    • Margarete Diaz-Cuadros
    • , Daniel E. Wagner
    •  & Olivier Pourquié
  • Article |

    Single-cell RNA sequencing of cells from healthy human liver, hepatocellular carcinoma and chimaeric mouse liver identifies subtypes of liver cells, epithelial progenitors and differences between healthy and diseased cells.

    • Nadim Aizarani
    • , Antonio Saviano
    •  & Dominic Grün
  • Letter |

    Transposon tagging to clonally trace progenitors and stem cells provides evidence for a substantially revised roadmap for unperturbed haematopoiesis, and highlights unique properties of multipotent progenitors and haematopoietic stem cells in situ.

    • Alejo E. Rodriguez-Fraticelli
    • , Samuel L. Wolock
    •  & Fernando D. Camargo
  • Letter |

    Exit of epiblasts from an unrestricted naive pluripotent state is required for epithelialization and generation of the pro-amniotic cavity in mouse embryos and for amniotic cavity formation in human embryos and human embryonic stem cells.

    • Marta N. Shahbazi
    • , Antonio Scialdone
    •  & Magdalena Zernicka-Goetz
  • Letter |

    A differentiation protocol to obtain enteric nervous system (ENS) progenitors and a range of neurons from human pluripotent stem cells is developed; the cells can migrate and graft to the colon of a chick embryo and an adult mouse colon, including in a mouse model of Hirschsprung disease, in which a functional rescue is observed.

    • Faranak Fattahi
    • , Julius A Steinbeck
    •  & Lorenz Studer
  • Letter |

    An analysis of mouse skin reveals that super-enhancers are critical to identity, lineage commitment and plasticity of adult stem cells; dynamic super-enhancer remodelling in new niches is dependent on the levels of pioneer transcription factor SOX9, which is identified as a key regulator of super-enhancer chromatin for hair follicle stem cells.

    • Rene C. Adam
    • , Hanseul Yang
    •  & Elaine Fuchs
  • Article
    | Open Access

    Lineage-specific transcription factors and signalling pathways cooperate with pluripotency regulators to control the transcriptional networks that drive cell specification and exit from an embryonic stem cell state; here, we report genome-wide binding data for 38 transcription factors combined with analysis of epigenomic and gene expression data during the differentiation of human embryonic stem cells into the three germ layers.

    • Alexander M. Tsankov
    • , Hongcang Gu
    •  & Alexander Meissner
  • Article |

    Through the use of a novel three-dimensional imaging technique, used in conjunction with a multicolour reporter that allows lineage tracing and cell tracking of entire mammary ducts in vivo, bipotent stem cells are shown to have a central role in both puberty and long-term maintenance; in addition, long-lived luminal progenitor cells with a prominent role in ductal expansion are identified.

    • Anne C. Rios
    • , Nai Yang Fu
    •  & Jane E. Visvader
  • Article |

    Transient overexpression of the transcription factors NKX2-1 and PAX8 in a murine cell model is shown to direct the differentiation of embryonic stem cells towards a thyroid follicular cell lineage; the resulting three-dimensional thyroid follicles created by subsequent thyrotropin treatment show hallmarks of thyroid function in vitro and rescue thyroid function in vivo when transplanted into athyroid mice, adding to our understanding of the molecular mechanisms underlying thyroid development.

    • Francesco Antonica
    • , Dominika Figini Kasprzyk
    •  & Sabine Costagliola
  • Letter |

    Polycomb proteins have a key role in regulating the expression of genes essential for development, differentiation and maintenance of cell fates. Here, Polycomb repressive complex 2 (PRC2) is shown to form a complex with JARID2, a Jumonji domain protein. JARID2 is required for the binding of Polycomb proteins to target genes in embryonic stem cells as well as for the proper differentiation of ES cells.

    • Diego Pasini
    • , Paul A. C. Cloos
    •  & Kristian Helin