RNA sequencing

Using spike-in controls with current single cell RNA-seq platforms remains a challenge. Here, the authors use a mixture of short, unmodified DNA oligos as a normalization standard for sci-RNAseq to improve the detection of global transcriptome changes.

Here, the authors demonstrate that single cell RNA sequencing technology can be leveraged to characterize RNA content of individual membrane-free condensates formed by liquid-liquid phase separation processes such as coacervation.

How fibrosis and inflammation are integrated is not entirely clear. Here the authors show that profibrotic proximal tubular cells in the kidneys recruit basophils and activate them to produce interleukin-6 and drive T_H17 cell responses.

The spatial context of epitranscriptomic features in the tumour microenvironment remains poorly understood. Here, a method for transcriptomic and epitranscriptomic analysis of immunofluorescence-stained tissue, Select-seq, is applied to stem cell-like microniches in triple negative breast cancer.