Protein design

Protein design (or protein engineering) is a technique by which proteins with enhanced or novel functional properties are created. Proteins can be engineered by rational design, which typically uses computational tools to identify useful mutations, or by directed evolution, which uses random mutagenesis coupled with a selection process to identify desired variants.

Latest Research and Reviews

News and Comment

  • Comments & Opinion |

    Deep learning has transformed protein structure modeling. Here we relate AlphaFold and RoseTTAFold to classical physically based approaches to protein structure prediction, and discuss the many areas of structural biology that are likely to be affected by further advances in deep learning.

    • Minkyung Baek
    •  & David Baker
    Nature Methods 19, 13-14
  • News & Views |

    In two seminal papers published 25 years ago, Phillips and Remington reported the structure of the green fluorescent protein (GFP). These studies provided a blueprint for the rational engineering of GFP, catalysing efforts that produced a large and growing collection of fluorescent proteins and indicators of cellular activity.

    • Jihwan Lee
    •  & François St-Pierre
  • News & Views |

    Genetic code expansion is emerging as a promising strategy to precisely regulate protein expression. A new study engineered cells that enabled noncanonical-amino-acid-triggered insulin expression to rapidly regulate blood glucose levels in a diabetic mouse model.

    • Jicheng Yu
    • , Yuqi Zhang
    •  & Zhen Gu
  • News & Views |

    The ability to control the subtle differences in reaction mechanisms and outcomes is an aspiration of many synthetic chemists. Now protein evolution has enabled the control of selectivity for hydroamination reactions catalysed by gold-based artificial metalloenzymes by favouring dual-gold catalysis over monomeric catalysis.

    • Amanda G. Jarvis
    Nature Catalysis 4, 639-640