Developmental biology

  • Article |

    A chromatin accessibility atlas of 240,919 cells in the adult and developing Drosophila brain reveals 95,000 enhancers, which are integrated in cell-type specific enhancer gene regulatory networks and decoded into combinations of functional transcription factor binding sites using deep learning.

    • Jasper Janssens
    • , Sara Aibar
    •  & Stein Aerts
  • Article |

    Following global DNA demethylation, mouse gonadal primordial germ cells undergo remodelling of repressive chromatin modifications, resulting in a sex-specific signature that is required to safeguard the transcriptional program.

    • Tien-Chi Huang
    • , Yi-Fang Wang
    •  & Petra Hajkova
  • Article
    | Open Access

    Blastoids derived from naive PXGL-cultured human pluripotent stem cells in which Hippo, TGF-β and ERK pathways are inhibited closely recapitulate aspects of blastocyst development, form cells resembling blastocyst-stage cells and thus provide a model system for implantation and development studies.

    • Harunobu Kagawa
    • , Alok Javali
    •  & Nicolas Rivron
  • Article |

    The single-cell transcriptional profile of a human embryo between 16 and 19 days after fertilization reveals parallels and differences in gastrulation in humans as compared with mouse and non-human primate models.

    • Richard C. V. Tyser
    • , Elmir Mahammadov
    •  & Shankar Srinivas
  • Article |

    In the post-mitotic nervous system of Caenorhabditis elegans, the promotion of a mature neuronal transcription program is controlled by the developmental-stage-specific upregulation of the microRNA LIN-4 and the subsequent repression of its target, the transcription factor lin-14.

    • HaoSheng Sun
    •  & Oliver Hobert
  • Article |

    Cryo-electron microscopy studies show that dynamic coordination of Na+ in the ion channel of Dispatched homologue 1 and the transmembrane Na+ gradient have key roles in exporting lipid-modified Hedgehog protein signal.

    • Qianqian Wang
    • , Daniel E. Asarnow
    •  & Philip A. Beachy
  • Article
    | Open Access

    Auxin induces transmembrane-kinase-dependent activation of H+-ATPase in the plasma membrane through phosphorylation of its penultimate threonine residue, promoting apoplastic acidification and hypocotyl cell elongation in Arabidopsis.

    • Wenwei Lin
    • , Xiang Zhou
    •  & Zhenbiao Yang
  • Article
    | Open Access

    RNA-sequencing analysis of the prenatal human brain at different stages of development shows that areal transcriptional signatures are dynamic and coexist with developmental and cell-type signatures.

    • Aparna Bhaduri
    • , Carmen Sandoval-Espinosa
    •  & Arnold R. Kriegstein
  • Article |

    A single-cell atlas of human fetal bone marrow in healthy fetuses and fetuses with Down syndrome provides insight into developmental haematopoiesis in humans and the transcription and functional differences that occur in Down syndrome.

    • Laura Jardine
    • , Simone Webb
    •  & Muzlifah Haniffa
  • Perspective |

    This Perspective outlines the Human Developmental Cell Atlas initiative, which uses state-of-the-art technologies to map and model human development across gestation, and discusses the early milestones that have been achieved.

    • Muzlifah Haniffa
    • , Deanne Taylor
    •  & Matthias Zilbauer
  • Article
    | Open Access

    Cells from embryonic, fetal, paediatric and adult human intestinal tissue are analysed at different locations along the intestinal tract to construct a single-cell atlas of the developing and adult human intestinal tract, encompassing all cell lineages.

    • Rasa Elmentaite
    • , Natsuhiko Kumasaka
    •  & Sarah A. Teichmann
  • Article |

    Serial-section electron microscopy is used to reconstruct the full brain connectome of eight individual Caenorhabditis elegans at various stages of development, providing insight into the principles underlying brain maturation.

    • Daniel Witvliet
    • , Ben Mulcahy
    •  & Mei Zhen
  • Article |

    A comprehensive single-cell transcriptomic atlas of the mouse brain between gastrulation and birth identifies hundreds of cellular states and reveals the spatiotemporal organization of brain development.

    • Gioele La Manno
    • , Kimberly Siletti
    •  & Sten Linnarsson
  • Article |

    Live imaging and single-cell transcriptomics of mouse hair follicles reveal their development from 2D concentric zones in the placode to 3D longitudinal compartments, one of which is a stem cell compartment.

    • Ritsuko Morita
    • , Noriko Sanzen
    •  & Hironobu Fujiwara
  • Article |

    Whole-genome sequencing of haematopoietic colonies from human fetuses reveals the somatic mutations acquired by individual progenitors, which are used as barcodes to construct a phylogenetic tree of blood development.

    • Michael Spencer Chapman
    • , Anna Maria Ranzoni
    •  & Ana Cvejic
  • Article |

    An aged, senescent immune system has a causal role in driving systemic ageing, and the targeting of senescent immune cells with senolytic drugs has the potential to suppress morbidities associated with old age.

    • Matthew J. Yousefzadeh
    • , Rafael R. Flores
    •  & Laura J. Niedernhofer
  • Review Article |

    This Review examines the evidence showing that DNA damage is associated with ageing phenotypes, suggesting that it may have a central role as the cause of ageing.

    • Björn Schumacher
    • , Joris Pothof
    •  & Jan H. J. Hoeijmakers
  • Article |

    In the eye lens of zebrafish and mice, the phospholipases Plaat1 and PLAAT3, respectively, are essential for macroautophagy-independent organelle degradation that produces an organelle-free zone and achieves optimal transparency.

    • Hideaki Morishita
    • , Tomoya Eguchi
    •  & Noboru Mizushima
  • Article |

    Fossiomanus sinensis and Jueconodon cheni, a newly described Early Cretaceous tritylodontid and eutriconodotan, respectively, show convergence on a fossorial body plan and provide insights into the evolution of the axial skeleton in mammaliamorphs.

    • Fangyuan Mao
    • , Chi Zhang
    •  & Jin Meng
  • Article |

    The duration of a critical period of plasticity in the developing Drosophila motor circuit, during which motor neurons display activity-dependent refinement of neurite structure and connectivity, is dependent on astrocyte to motor neuron Neuroligin–Neurexin signalling.

    • Sarah D. Ackerman
    • , Nelson A. Perez-Catalan
    •  & Chris Q. Doe
  • Article |

    Phylogenies of human placental cells based on whole-genome sequencing of bulk samples and microdissections reveal extensive mutagenesis in placental tissue, and suggest that mosaicism is a typical part of normal placental development.

    • Tim H. H. Coorens
    • , Thomas R. W. Oliver
    •  & Sam Behjati
  • Article |

    Mechanical load-sharing enables the long-range cooperative uptake of apoptotic cells by multiple epithelial cells; and clearance of these apoptotic cells facilitates error correction, which is necessary for developmental robustness and survival of the embryo.

    • Esteban Hoijman
    • , Hanna-Maria Häkkinen
    •  & Verena Ruprecht
  • Article |

    Primed pluripotent stem cells from distant species compete with each other, and inactivation of NF-κB signalling in normally outcompeted human cells improves their survival and chimerism in mouse embryos.

    • Canbin Zheng
    • , Yingying Hu
    •  & Jun Wu
  • Article |

    The rate of scale regeneration in zebrafish is controlled by the frequency of rhythmic travelling waves of Erk activity, which are broadcast from a central source to induce ring-like patterns of osteoblast tissue growth.

    • Alessandro De Simone
    • , Maya N. Evanitsky
    •  & Stefano Di Talia
  • Article |

    Lymphatic endothelium secretes factors needed for heart growth and repair such as RELN, which helps with heart regeneration and cardioprotection after myocardial infarction.

    • Xiaolei Liu
    • , Ester De la Cruz
    •  & Guillermo Oliver
  • Article |

    Arterial development relies on the timely and MYC-dependent suppression of endothelial metabolism and the cell cycle in pre-arterial endothelial cells through Notch signalling.

    • Wen Luo
    • , Irene Garcia-Gonzalez
    •  & Rui Benedito
  • Article |

    Differences in the mechanical properties of individual cardiomyocytes drive their segregation into compact versus trabecular layer, thereby transforming the myocardium in a developing heart from a simple epithelium into an intricately patterned tissue with distinct cell fates.

    • Rashmi Priya
    • , Srinivas Allanki
    •  & Didier Y. R. Stainier