Cellular noise

  • Letter |

    Through drug exposure, a rare, transient transcriptional program characterized by high levels of expression of known resistance drivers can get ‘burned in’, leading to the selection of cells endowed with a transcriptional drug resistance and thus more chemoresistant cancers.

    • Sydney M. Shaffer
    • , Margaret C. Dunagin
    •  & Arjun Raj
  • Letter |

    An algorithm that allows rare cell type identification in a complex population of single cells, based on single-cell mRNA-sequencing, is applied to mouse intestinal cells, revealing novel subtypes of enteroendocrine cells and showing that the Lgr5-expressing population consists of a homogenous stem cell population with a few rare secretory cells, including Paneth cells.

    • Dominic Grün
    • , Anna Lyubimova
    •  & Alexander van Oudenaarden
  • Letter |

    A single-cell method for probing genome-wide chromatin accessibility has been developed; the results provide insight into the relationship between cell-to-cell variation associated with specific trans-factors and cis-elements, as well insights into the relationship between chromatin accessibility and three-dimensional genome organization.

    • Jason D. Buenrostro
    • , Beijing Wu
    •  & William J. Greenleaf
  • Letter |

    Quantification of single-cell growth over long periods of time in E. coli shows transient oscillations in cell size, with periods stretching across more than ten generations; a noisy negative feedback on cell-size control is proposed in which cells with a small initial size tend to divide later than cells with a large initial size with implications for the genetic and physiological processes required.

    • Yu Tanouchi
    • , Anand Pai
    •  & Lingchong You
  • Letter |

    Precise measurement of cell-cycle duration in thousands of mammalian cells reveals correlations among cousin cells, but no such correlations between mother and daughter cells; recapitulating this finding using a deterministic model suggests that observed cellular heterogeneities in cell-cycle duration may be attributable to deterministic processes, and eventually be controlled.

    • Oded Sandler
    • , Sivan Pearl Mizrahi
    •  & Nathalie Q. Balaban