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Back and forth with nanopore peptide sequencing

Tagging peptides with DNA allows repeated reads via a helicase in a nanopore with reduced error rates.

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Fig. 1: A reading–rereading molecular machine for single-molecule peptide detection.


  1. Brinkerhoff, H., Kang, A. S. W., Liu, J., Aksimentiev, A. & Dekker, C. Science 374, eabl4381 (2021).

    Article  Google Scholar 

  2. Department of Health and Human Services. RFA-HG-21-006: transformative nucleic acid sequencing technology innovation and early development (R01 clinical trial not allowed). (accessed 8 January 2022).

  3. T2T Consortium. (accessed 8 January 2022).

  4. Kelly, R. T. Mol. Cell. Proteomics 19, 1739–1748 (2020).

    CAS  Article  Google Scholar 

  5. Yan, S. et al. Nano Lett. 21, 6703–6710 (2021).

    CAS  Article  Google Scholar 

  6. Chen, Z. et al. Chem. Sci. (2021).

  7. Yu, L. et al. Preprint at bioRxiv (2021).

  8. Nivala, J., Marks, D. B. & Akeson, M. Nat. Biotechnol. 31, 247–250 (2013).

    CAS  Article  Google Scholar 

  9. Ouldali, H. et al. Nat. Biotechnol. 38, 176–181 (2020).

    CAS  Article  Google Scholar 

  10. Lucas, F. L. R., Versloot, R. C. A., Yakovlieva, L., Walvoort, M. T. C. & Maglia, G. Nat. Commun. 12, 5795 (2021).

    CAS  Article  Google Scholar 

  11. Zhang, S. et al. Nat. Chem. 13, 1192–1199 (2021).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Meni Wanunu.

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Wanunu, M. Back and forth with nanopore peptide sequencing. Nat Biotechnol 40, 172–173 (2022).

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