Extended Data Fig. 1: Identification of a TERT-high subpopulation in acinar cells and beta cells but not duct cells using lineage tracing. | Nature

Extended Data Fig. 1: Identification of a TERT-high subpopulation in acinar cells and beta cells but not duct cells using lineage tracing.

From: Acinar cell clonal expansion in pancreas homeostasis and carcinogenesis

Extended Data Fig. 1

a, TertCreERT2/+; Rosa26LSL-tdTomato/+ mouse, which responds to tamoxifen-induced Cre-mediated activation of the tdTomato reporter allele. b, Immunohistochemistry analysis of Tomato in a TertCreERT2/+; Rosa26LSL-tdTomato/+ (TertCreER) pancreas after oil and tamoxifen injection (n=4 mice for oil ctrl; n=11 mice for tamoxifen). Mo, month. c, d, Co-immunofluorescence stains 10 days after single dose of tamoxifen (5mg) in TertCreER pancreas (n=4 mice). Tomato (red, c, d), DAPI (blue, c, d), Insulin (grey, c, d) Sox9 (green, c) and Amylase (green, d) are shown. e, f, Single-molecule RNA FISH for Tert mRNA (e) on FACS-purified bulk acinar cells (e, f) from TertCreER mice 10d after Tamoxifen (5mg) injection (n=4 mice). g, Quantification of Tomato-positive cells in TertCreER mice in the acinar cell compartment 10 days and within pancreatic ductal cells 10 days and 12 months post tamoxifen activation (n=5 mice). h, qRT-PCR for indicated genes in FACS-sorted TomNeg (white bars) and TomPos (grey bars) acinar cells. i, j, Immunofluorescence analysis of a TertCreER pancreas 10 days after a single dose of 5mg tamoxifen (n=5 mice). Tomato (red), Dclk1 (i, green) or BMI1 (j, green) and DAPI (blue) are shown. Scale bar, 100µm (b), 50µm (e, f) and 25µm (c, d, i, j). All data are mean± SD. P values calculated by two-sided Mann-Whitney test (h).

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