Extended Data Fig. 8: Effect of Arg66→Gln substitution on BuMiaB activity. | Nature

Extended Data Fig. 8: Effect of Arg66→Gln substitution on BuMiaB activity.

From: Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB

Extended Data Fig. 8

a, Time course for SAH formation upon incubating 25 μM BuMiaB WT (black circles) or BuMiaB R66Q (red circles) with 1 mM SAM in the absence of dithionite. b-e, Time course for formation of SAH (b), 5’dAH (c), ms2i6A (d), and decay of i6A (e), after 30 min of initial incubation of 25 μM BuMiaB with 1 mM SAM followed by addition of 100 μM i6A ACSL RNA and reaction initiation with 1 mM dithionite. The black colour corresponds to data obtained for BuMiaB WT in the presence of the 17-mer RNA substrate; the blue colour corresponds to data obtained in the presence of the 13-mer; and the red colour corresponds to data for BuMiaB R66Q in the presence of the 17-mer. Error bars represent one standard deviation for reactions conducted in triplicate, with the centre representing the mean.

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