Extended Data Fig. 2: UTX cIDR and the key residues in cIDR are important for forming condensates and its tumour suppressive activity. | Nature

Extended Data Fig. 2: UTX cIDR and the key residues in cIDR are important for forming condensates and its tumour suppressive activity.

From: UTX condensation underlies its tumour-suppressive activity

Extended Data Fig. 2

a. Growth curves of THP-1 cells electroporated with indicated constructs, shown as mean ± SD. n = 3 independent experiments. P value is between MT2 and ΔcIDR at the last time point. b. Immunoblotting by indicated antibody of total lysates from THP-1 cells electroporated with empty vector or indicated UTX WT or mutants. c. Fluorescence images of THP-1 cells electroporated with indicated UTX constructs fused to EGFP. d. Analysis of amino acid enrichment for UTX cIDR. By Composition Profiler, using indicated background sample. e. Fluorescence images of 293T cells transfected with the corresponding UTX WT or mutants fused with EGFP. f. Immunoblotting by indicated antibody of total lysates from 293T cells transfected with empty vector or indicated UTX WT or mutants fused to EGFP. g. Immunoblotting by indicated antibody of total lysates from THP-1 cells electroporated with empty vector or indicated UTX WT or mutants. h. Representative fluorescence microscopy images of foci in FRAP assays for full-length UTX WT and HY fused to mEGFP in nuclei following transfection into 293T cells. The photobleached focus is indicated by the dashed box and its images at indicated time points are amplified. in all used 20 µM mEGFP, mEGFP-UTX (419–848) WT or HY as indicated. i. Pictures and OD600 plotted as mean ± SD. n = 3 independent experiments. j. Representative fluorescence microscopy and DIC images at indicated times in condensation. Scale bar, 10 µm. k. FRAP recovery curves as mean ± SD at indicated early and late times in condensation. n = 13 and 12 droplets each for WT and HY, respectively, for early times, and n = 8 and 12 droplets each for WT and HY, respectively, for late times. ln. Line RICS assays for WT (n = 32 droplets) and HY (n = 12) over the time range of 0-100 min in condensation. l. Fluorescence Images and line carpets. m. Line RICS autocorrelation curves. n. Plot showing the mean ± SD of the diffusion coefficients. *P<0.05, ***P<0.001, and ****P<0.0001, by two-sided t-test for all.

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