Extended Data Fig. 6: Purified UTX and MLL4 or p300 regions form co-condensates through specific interaction mediated by TPR. | Nature

Extended Data Fig. 6: Purified UTX and MLL4 or p300 regions form co-condensates through specific interaction mediated by TPR.

From: UTX condensation underlies its tumour-suppressive activity

Extended Data Fig. 6

a. Coomassie blue staining image of purified EGFP-UTX (419-848), EGFP-UTX (1-848), and mCherry-MLL4C(N). b. Fluorescence images of purified proteins individually diluted to indicated concentrations. c. Fluorescence images of purified proteins mixed and diluted to the final concentration as indicated, equal to the concentrations in b. d. Fluorescence images of purified proteins individually diluted to indicated concentrations. e. Fluorescence images of purified proteins mixed and diluted to the final concentration equal to that of the corresponding protein in d. The exposure time for GFP in the top row was longer than those in the other two rows to adjust the GFP brightness due to the much lower concentration of the GFP-fused proteins in the top row. f. Recruitment coefficient for MLL4C(N) was determined as the relative ratio of the mCherry signal amount inside the GFP condensates over that inside the mCherry condensates, and plotted as mean ± SD. n = 3 independent fields each. Note that each field had ~100 total droplets. **P<0.01 by one-way ANOVA followed by Tukey’s post hoc test. g. Domain schematic of p300. P300 (251-550) containing ZF1 domain is labeled. h. Fluorescence images of purified proteins mixed to the indicated final concentrations.

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