Extended Data Fig. 5: UTX co-condenses with and enhance chromatin recruitment of MLL4 and p300 in cells. | Nature

Extended Data Fig. 5: UTX co-condenses with and enhance chromatin recruitment of MLL4 and p300 in cells.

From: UTX condensation underlies its tumour-suppressive activity

Extended Data Fig. 5

a. A schematic showing the domain compositions of MLL4, MLL4f, and MLL4C(N). b. Left, a schematic showing the optoDroplet system used for the right, which are confocal images of 293T cells expressing MLL4C(N)-mCherry-Cry2, before and after light activation. c. Left, a schematic showing the optoDroplet system used for the right, which are confocal images of 293T cells expressing UTX-mCherry-Cry2, before and after light activation. d. Left, a schematic showing the optoDroplet system used for the right, which are confocal images of 293T cells expressing both UTX (WT or indicated mutant)-mCherry-Cry2 and MLL4C(N)-EGFP, before and after light activation. e. Left, a schematic showing the LacO-array system. Middle, fluorescence microscopy images of LacO-cells transfected with EGFP-LacI (control) or UTX (WT or mutant)-EGFP-LacI, each together with p300(251-550)-mCherry. Right, recruitment coefficients of p300(251-550) relative to the LacO-bound control or UTX (WT or mutant) are determined as described in Methods and plotted as average ± SD. n = 11, 19, 17, 24 cells for control, WT, ΔcIDR, and ΔTPR, respectively. P values by one-way ANOVA followed by Tukey’s post hoc test.

Back to article page