Extended Data Fig. 1: Compound-dependent bacterial morphologies and evidence of porin overexpression in TOMAS. | Nature

Extended Data Fig. 1: Compound-dependent bacterial morphologies and evidence of porin overexpression in TOMAS.

From: Rational design of a new antibiotic class for drug-resistant infections

Extended Data Fig. 1

a, Morphology of P. aeruginosa PAO1 without drug treatment (left panel, 5 µm scale bar) or treated with 0.5X MIC of either NXL-105 (middle panel, 5 µm scale bar) or Compound 2 (right panel, 10 µm scale bar). Micrographs are representative of results obtained for three biologically independent studies. b, SDS-PAGE analysis of overexpressed porins in TOMAS19 after overnight induction with 120 µM L-arabinose. 2 µg outer membrane extracts were loaded onto a 13% polyacrylamide-SDS gel in 6 M urea. Expression levels are comparable to those observed for OprD in TOMAS19. Gel is representative of two biologically independent studies. Bands corresponding to the overexpressed porins are highlighted in boxes. Upper panel = P. aeruginosa porins; lower panel = K. pneumoniae porins.

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