Extended Data Fig. 7: Drug repurposing can overcome T790M-like resistance mutations. | Nature

Extended Data Fig. 7: Drug repurposing can overcome T790M-like resistance mutations.

From: Structure-based classification predicts drug response in EGFR-mutant NSCLC

Extended Data Fig. 7

a, Heat map with unsupervised hierarchical clustering of log (mutant/WT) ratios from Ba/F3 cells expressing indicated mutations after 72 h of indicated drug treatment. Squares are representative of the median of n=3 replicates. For co-occurring mutations, the order of mutations 1, 2, and 3 were assigned arbitrarily. Groups were assigned based on hierarchal clustering and known resistance mutations. b, c, Dot plot of mutant/WT IC50 values of Ba/F3 cells expressing T790M-like-3S (3rd-gen TKI sensitive) (b) and T790M-like-3R (3rd-gen TKI resistant) (c) treated with indicated classes of EGFR TKIs. Dots are representative of average of n=3 replicate mutant/WT IC50 values of individual cell lines with individual drugs and drugs of each class are grouped together. Bars are representative of average mutant/WT IC50 values ± SEM for each class of EGFR TKI and all cell lines. p-values were determined by one-way ANOVA with unequal variance as determined by Brown-Forsythe test to determine differences in variance. Holm-Sidak’s multiple comparisons test was used to determine differences between groups

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