Extended Data Fig. 5: Comparison of actions of H9T versus natural cytokines in CD8+ T cells. | Nature

Extended Data Fig. 5: Comparison of actions of H9T versus natural cytokines in CD8+ T cells.

From: An engineered IL-2 partial agonist promotes CD8+ T cell stemness

Extended Data Fig. 5

a, b, Pre-activated mouse (a) or human (b) CD8+ T cells were rested and cultured with 10 nM of the indicated cytokines for 6 days, and cell density was counted by beads-based flow cytometry. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction. a, n = 6 mice; b, n=6 donors. Data are representative of two independent experiments. c, Pre-activated mouse CD8+ cells were rested and cultured with 10 nM of the indicated cytokines for 6 days, and TIM-3 expression was examined by flow cytometry. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction, n = 4 mice. Data are representative of two independent experiments. df, Pre-activated mouse CD8+ T cells were rested overnight and cultured with 10 nM of the indicated cytokines for 0, 0.5, 1, 2, or 4 h. Cells were then fixed, permeabilized, and stained for STAT5-pY694 (d), AKT-pS473 (e) and ERK-pT202/pY204 (f). Data are mean ± SEM, n = 6 mice, Data are representative of two independent experiments. g, h, Pre-activated mouse CD8+ T cells were rested and cultured with 10 nM of the indicated cytokines for 1 day and collected for RNA-seq. PCA analysis (g) and selected gene expression (h) are shown. Data are combined from two biological repeats

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