Extended Data Fig. 2: Functional analysis of CD8+ T cells expanded with IL-2, H9 or H9T. | Nature

Extended Data Fig. 2: Functional analysis of CD8+ T cells expanded with IL-2, H9 or H9T.

From: An engineered IL-2 partial agonist promotes CD8+ T cell stemness

Extended Data Fig. 2

a, b, Expression of CD62L and the percentage of memory-like cells (CD62L+CD44+). Pre-activated mouse CD8+ T cells were cultured with 10 nM IL-2, H9, or H9T for 8 days, and stained for CD44 and CD62L. Data are mean values ± SEM, n = 6 mice, one-way ANOVA test with Dunnett’s correction. Data are representative of three independent experiments. c, Expression of CD62L in 8 day IL-2- or H9T-cultured cells or cells cultured for 6 days in H9T-containing medium and then switched to IL-2-containing medium for 2 days. Pre-activated CD8+ T cells were cultured with 10 nM IL-2 or H9T, and on day 6, a fraction of the H9T-expanded cells was washed and subsequently cultured with 10 nM IL-2. Cells were collected two days later and analysed by flow cytometry. Data are presented as mean values ± SEM, n = 4 mice, one-way ANOVA test with Dunnett’s correction. Data are representative of two independent experiments. dg, Cytokine production and memory population in CD8+ T cells expanded with IL-2, H9, or H9T. Pre-activated CD8+ T cells were expanded for 8 days with IL-2, H9, or H9T, stimulated with 100 nM gp100 or control peptide for 1 h, and then treated with 5 µg/ml Brefeldin A for 5 h. Cells were then fixed for intracellular staining of IFN-γ, n = 5 mice (d); TNF, n = 5 mice (e); IL-2, n = 3 mice (f); and IL-10, n = 5 mice (g); Data are presented as mean values ± SEM, one-way ANOVA test with Dunnett’s correction. Data are representative of two independent experiments

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