Extended Data Fig. 7: Dose response of IL-2, IL-15 and H9T in CD8+ T cells. | Nature

Extended Data Fig. 7: Dose response of IL-2, IL-15 and H9T in CD8+ T cells.

From: An engineered IL-2 partial agonist promotes CD8+ T cell stemness

Extended Data Fig. 7

ac, Dose response of IL-2, IL-15, and H9T in human CD8+ T cells. Pre-activated human CD8+ cells were rested and cultured with 0-100 nM of IL-2, IL-15, or H9T for 6 days and stained for surface expression of TIM-3 (a) or permeabilized and stained for intracellular granzyme B (b). Cell expansion rate (c) was assessed using flow cytometry based counting beads. Data are mean ± SEM, n=2 donors. Data are representative of two independent experiments. df, mRNA levels of TCF7, CD27 and SLC2A1 in human CD8+ T cells. Pre-activated human CD8+ cells were rested and stimulated with 1 nM of IL-2, IL-15, or H9T for 24 h. Cells were collected and mRNA extracted for qPCR analysis of TCF7 (d), CD27 (e) and SLC2A1 (f). The mRNA expression was normalized to that of RPLP0. Data are representative of two independent experiments

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