Extended Data Fig. 2: Synthetic lethal effects of lenvatinib and EGFR inhibitors on liver cancer cells expressing a high level of EGFR in vitro. | Nature

Extended Data Fig. 2: Synthetic lethal effects of lenvatinib and EGFR inhibitors on liver cancer cells expressing a high level of EGFR in vitro.

From: EGFR activation limits the response of liver cancer to lenvatinib

Extended Data Fig. 2

af, EGFRhigh liver cancer cell lines (SNU449, JHH1, Huh6 and SNU182) or EGFRlow cells (SNU398 and HepG2) were treated with lenvatinib, EGFR inhibitors (gefitinib or erlotinib) or their combination at the indicated concentrations. Growth curves were determined by Incucyte live cell analyses. Data are mean ± s.e.m., n = 5. gl, The quantification of three independent assays in Fig. 2b–g. Data are mean ± s.e.m., n = 3 independent experiments. mo, Ectopic EGFR expression confers resistance to lenvatinib in EGFRlow cells. m, Western blot analysis of p-EGFR and total EGFR levels in SNU398 and HepG2 cells infected with control or EGFR overexpression vector. HSP90 served as a control. n, o, SNU398 and HepG2 cells expressing control vector or EGFR were cultured with or without lenvatinib (SNU398, 5 μM; HepG2, 10 μM). n, The cells were fixed, stained and photographed after 14 days. o, Quantification of three independent assays. Data are mean ± s.e.m., n = 3 independent experiments

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