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Reply to: Zebrafish prrx1a mutants have normal hearts

The Original Article was published on 23 September 2020

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Fig. 1: prrx1a-crispant embryos show mesocardia and a smaller atrium without early defects in the LRO.
Fig. 2: EMT transcription factors in heart laterality in zebrafish.

Data availability

All of the raw data that support the findings of this study are available within the manuscript and its associated files. Source data are provided with this paper.


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Work in the laboratory is supported by grants from the Ministry of Science and Innovation through the Spanish State Research Agency (AEI) (MICIU RTI2018-096501-B-I00) and from Generalitat Valenciana (PROMETEOII/2017/150). M.A.N. also acknowledges the ‘Severo Ochoa’ Programme for Centres of Excellence in R&D (SEV-2017-0273) to Instituto de Neurociencias.

Author information




N.C. performed the majority of the experiments, analysed the data and prepared the figures; L.R. performed CRISPR injections and collaborated on the scoring of heart positioning; and A.A. performed bioinformatics analyses. N.C., L.R. and A.A. did not contribute to the original publication but they contributed to this Matters Arising, mainly because O.H.O. and H.C., the main authors of the previous study, moved to the National Centre for Cardiovascular Diseases (CNIC, Spain) and Harvard University, respectively. C. Minguillón, P. Murawala, E. M. Tanaka and R. Muñoz-Chápuli, the other co-authors of the previous study1, did not contribute to the issues raised in this Reply. They have been informed, and agreed. J.G. designed the CRISPR guides and performed the mutant selection. M.A.N. conceived the study, interpreted the data and wrote the manuscript.

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Correspondence to M. Angela Nieto.

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Castroviejo, N., Ocaña, O.H., Rago, L. et al. Reply to: Zebrafish prrx1a mutants have normal hearts. Nature 585, E17–E19 (2020).

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