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Structure of HIV-1 Vpr in complex with the human nucleotide excision repair protein hHR23A


HIV-1 Vpr is a prototypic member of a large family of structurally related lentiviral virulence factors that antagonize various aspects of innate antiviral immunity. It subverts host cell DNA repair and protein degradation machineries by binding and inhibiting specific post-replication repair enzymes, linking them via the DCAF1 substrate adaptor to the Cullin 4 RING E3 ligase (CRL4DCAF1). HIV-1 Vpr also binds to the multi-domain protein hHR23A, which interacts with the nucleotide excision repair protein XPC and shuttles ubiquitinated proteins to the proteasome. Here, we report the atomic resolution structure of Vpr in complex with the C-terminal half of hHR23A, containing the XPC-binding (XPCB) and ubiquitin-associated (UBA2) domains. The XPCB and UBA2 domains bind to different sides of Vpr’s 3-helix-bundle structure, with UBA2 interacting with the α2 and α3 helices of Vpr, while the XPCB domain contacts the opposite side of Vpr’s α3 helix. The structure as well as biochemical results reveal that hHR23A and DCAF1 use overlapping binding surfaces on Vpr, even though the two proteins exhibit entirely different three-dimensional structures. Our findings show that Vpr independently targets hHR23A- and DCAF1- dependent pathways and highlight HIV-1 Vpr as a versatile module that interferes with DNA repair and protein degradation pathways.


Vpr is one of the four HIV-1 accessory virulence factors, Vif, Vpr, Vpu, and Nef, that antagonize innate mechanisms and intrinsic cellular pathways that interfere with effective viral replication1,2,3,4,5,6,7. Closely structurally related orthologues and paralogues of HIV-1 Vpr are found in HIV-2 and essentially all primate lentiviruses8,9,10. While our knowledge of how Vpr promotes HIV-1 replication and pathogenesis is clearly incomplete, one well-established mechanism involves hijacking of the CRL4 E3 ubiquitin ligase and its DCAF1 substrate receptor (CRL4DCAF1) for depletion of cellular proteins that directly or indirectly target viral components. In particular, Vpr binds to DCAF1 and mediates loading of different protein substrates onto this E3 ligase, leading to their polyubiquitination and proteasome-dependent degradation11,12,13. Intriguingly, among these substrates are proteins involved in post-replication DNA repair and chromatin metabolism, including the base excision repair (BER) protein Uracil-DNA glycosylase (UNG2)14,15, the double strand break repair and Holliday junction resolvase MUS81-EME116,17, the helicase-like transcription factor (HLTF) that plays a role in the repair of stalled replication forks18,19,20, Exo1, a nuclease involved in double strand break repair21, and TET2, a methylcytosine dioxygenase that is a potent epigenetic regulator of gene expression in hematopoietic cells22. The structural basis of E3 ligase targeting by Vpr was elucidated for the BER protein UNG2: the substrate receptor DCAF1 engages helix α3 and, on the opposing surface of Vpr, a loop between helices α2 and α3 inserts into the active site of UNG2, thereby interfering with DNA binding and catalysis23. Significantly, UNG2, Exo1, HLTF and TET2 were shown to interfere with HIV-1 infection, supporting the notion that Vpr antagonizes specific post-replication DNA repair proteins that restrict HIV-1, thereby facilitating HIV-1 replication4,21,22,24.

HIV-1 Vpr also binds tightly to hHR23A25,26,27, the human homolog of yeast Rad23, which not only partakes in nucleotide excision repair (NER) but also plays important roles in various other aspects of cellular metabolism, beyond DNA repair28. hHR23A and its hHR23B isoform are modular and comprise four independently folded domains, joined by three flexible linkers. In particular, they possess an N-terminal ubiquitin-like domain (UBL), a central ubiquitin-associated domain (UBA1), followed by a RAD4/XPC (xeroderma pigmentosum group C) binding domain (RBD/XPCB) and a C-terminal ubiquitin-associated domain (UBA2). hHR23 proteins participate in NER by forming a complex with the XPC protein, mediated by the XPCB domain, and play a role in the initial recognition of damaged sites in DNA29. In addition, the hHR23B–XPC complex was shown to function as a co-activator controlling particular transcription programs30,31,32. The UBL domain mediates 26S proteasome binding by engaging the Rpn1 and Rpn10 subunits33,34, while the UBA domains capture polyubiquitin-conjugated target proteins, especially Lys-48-conjugated chains35.

Previously, we and others demonstrated that Vpr, which comprises a three-helix bundle with flexible N- and C-terminal tails23,36, binds the C-terminal half of hHR23A, contacting the UBA225 and the XPCB37 domains. Interestingly, in contrast to other DNA repair proteins that are targeted by Vpr, hHR23A does not become ubiquitinated in a Vpr-dependent manner and is not depleted in cells expressing HIV-1 Vpr21, suggesting that hHR23A is not a Vpr-recruited CRL4DCAF1 E3 substrate and that the Vpr–hHR23A complex likely presents a distinct architecture.

Here, we report the high-resolution NMR structure of Vpr, complexed with the C-terminal half of hHR23A, containing the XPCB and UBA2 domains, and a 2.2 Å resolution X-ray structure of the Vpr/UBA2 domain complex. Interestingly, our structural findings reveal that hHR23A interacts with the DCAF1-binding and not the substrate-binding Vpr surface, unlike other previously characterized Vpr targets involved in post-replication DNA repair pathways, and that this interaction antagonizes DCAF1 binding. We also show that Vpr binds hHR23A in a complex with XPC. Thus, our findings establish that HIV-1 Vpr interfaces with DNA repair pathways not only via CRL4DCAF1 E3 and proteasomal degradation of targets, but also through the hHR23/XPC complex, suggesting the possibility of an alternative to the ubiquitin ligase route. Our findings further illustrate how HIV-1 Vpr acts as a versatile structural adapter that targets diverse DNA repair pathways.


Vpr/hHR23A complex preparation and NMR and X-ray studies

Previous work from our laboratory demonstrated that the C-terminal half of hHR23A, hHR23A223–363, comprising both XPCB and UBA2 domains, directly binds Vpr37. Although the Vpr1–79/hHR23A223–363 complex could be prepared after co-expression, purification in amounts for structural studies proved challenging, and screening by 1H–15N HSQC NMR spectroscopy revealed a mixture of complex and free hHR23A223–363 proteins37. Therefore, in order to prepare a homogeneous sample of the complex, we generated Vpr1–79 and hHR23A223–363 fusion constructs, connecting Vpr to either the N- or C-terminus of the double domain construct of hHR23A by a six amino acid (GGS)2 linker (L) (details are provided in the “Methods” section). Assessment of the structural properties of the linked proteins was performed by 1H–15N HSQC spectroscopy. While the spectrum of the C-terminal fusion construct, hHR23A223–363–L-Vpr1–79, exhibited severe NMR line-broadening and loss of 1H–15N HSQC cross peaks (Supplementary Fig. 1a) compared to the free hHR23a protein, the spectrum of the N-terminal fusion construct, Vpr1–79–L-hHR23A223–363 (Supplementary Fig. 1b), was of sufficient quality to proceed with resonance assignments and structure determination. This suggested that the six amino acid containing linker at the end of Vpr1–79 was long enough to allow for correct positioning of the two proteins in the complex, while it was most likely too short to permit correct complex formation when used to connect the C-terminus of hHR23A to the N-terminus of Vpr. Good agreement for the amide resonances in the 1H–15N HSQC spectrum of the Vpr1–79–L-hHR23A223–363 linked and the Vpr1–79 and hHR23A223–363 co-expressed complexes are noted (Supplementary Fig. 1c), confirming that essentially identical complexes are formed by the linked and unlinked proteins.

NMR assignments for the linked Vpr1–79–L-hHR23A223–363 construct, in which Vpr is intramolecularly bound to hHR23A223–363, were obtained for 97% of the backbone resonances and 92% of all side chain resonances. Chemical shift-derived secondary structure elements for Vpr and the XPCB and UBA2 domains (Supplementary Fig. 1d) are essentially the same as those for free Vpr36 and the individual hHR23A domains38,39,40. The amide resonances for the 13 N-terminal Vpr residues, those of the (GGS)2 linker, the first seven residues (223–229) before XPCB, the flexible linker residues (288–314) between XPCB and UBA2 and the last residues (360–363) following UBA2 are sharp and exhibit only small secondary shifts, compared to random coil values (Supplementary Fig. 1d), indicating that these regions are mobile and essentially adopt random coil conformations. Comparison between assignments for the Vpr-bound portion of hHR23A in Vpr1–79–L-hHR23A223–363 and free hHR23A223–363 permitted unequivocal delineation of the Vpr binding site on hHR23A by chemical shift mapping. This refined and completed the details of our earlier binding site determination37, which for the non-linked Vpr-complexed hHR23A had been largely based on comparing amide backbone assignments. We observed significant chemical shift differences (CSDs) (>0.2 ppm) for resonances associated with all helices, but α1, in the XPCB domain, confirming that most of XPCB is involved in binding. Even larger CSDs (>0.3 ppm) were observed for amide resonances belonging to the UBA2 domain, in particular for resonances associated with helices α2 and α3. Resonances of amino acids in the first helices of both, the XPCB and UBA2 domains, exhibited only very small CSDs, indicating that they are marginally affected by the interaction with Vpr. The magnitude and organization of all CSDs along the hHR23A223–363 amino acid sequence are summarized in Fig. 1a.

Fig. 1: Interaction of hHR23A and Vpr in the Vpr1–79–L-hHR23A223–363 complex.
figure 1

a Mapping of the Vpr binding site on hHR23A223–363 in the Vpr1–79–L-hHR23A223–363 complex by NMR. The 1H,15N-combined chemical shift differences (CSDs), calculated using the equation, (ΔδHN2 + (ΔδN/6)2)1/2 (for all amino acids, but prolines) or |Δδ| (for prolines), with ΔδHN, ΔδN and Δδ representing the 1HN, 15N and Hα chemical shift differences between resonance frequencies of free hHR23A223–363 and those in the Vpr1–79–L-hHR23A223–363 complex, are plotted along the linear amino acid sequence. The average CSD value is drawn in a red dotted line. Domain and secondary structure elements are schematically indicated at the top. b, c Lowest energy NMR conformer of the Vpr1–79–L-hHR23A223–363 complex in ribbon representation (b) and worm (Vpr1–79) and space filling representation (hHR23A223–363) (c). d Close-up view of the Zn2+-binding motif. Distances between the Zn2+ ion and His and Cys atoms are labeled. Helices are labeled as α1, α2 and α3.

Crystallization of Vpr1–79–L-hHR23A223–363 yielded crystals belonging to the P3121 space group. Surprisingly, no electron density could be located for the XPCB domain, most likely resulting from its loss due to proteolytic cleavage during crystallization. Mass spectrometry of the crystals confirmed the presence of the UBA2 domain but provided no evidence for the presence of the XPCB domain (Supplementary Fig. 2). Crystals contained four Vpr and two hHR23A-UBA2 molecules in the asymmetric unit, and the structure was solved using the phases from the native Vpr Zn2+ anomalous signal, collected at the Zn2+ edge (9.65 keV) to 3.0 Å resolution and further extended to 2.2 Å from a dataset collected at the Se edge (12.65 keV). The model was refined using the program BUSTER41,42, followed by manual model building in Coot43, to a final Rwork/Rfree value of 19.7/21.6%, respectively (see Table 1).

Table 1 Data collection and refinement statistics.

Overall structure of the Vpr/hHR23A complex

The NMR solution structure of the linked Vpr1-79–L-hHR23A223–363 complex (Fig. 1b–d) was calculated on the basis of 7650 NOE-derived distance constraints, including 81 Vpr/XPCB and 257 Vpr/UBA2 intermolecular distance constraints, and 330 dihedral angle constraints. The final 55-conformer ensemble of the complex satisfies all experimental constraints and displays excellent covalent geometry with atomic root-mean-square differences (r.m.s.d.) of 0.67 ± 0.06 and 1.13 ± 0.05 Å for the backbone and all heavy atoms, respectively, relative to their mean position (Table 2 and Supplementary Fig. 1e). The Vpr structure consists of three anti-parallel α-helices packed into a helical bundle, and very few conformational changes are noted in the hHR23A-bound Vpr, compared to the free structure36. Vpr’s helix α3 is buried between the XPCB and UBA2 domains (Fig. 1b, c). Further interactions in the complex involve Vpr’s α2 and UBA2’s α3 helices. In the X-ray structure of the Vpr/UBA2 domain complex, the features of the interaction interface are very similar to those observed in the NMR structure: the backbone r.m.s.d. between the NMR and X-ray structures for residues 14–78 of Vpr and residues 315–359 of UBA2 is 1.07 Å (Supplementary Fig. 1f).

Table 2 Statistics for the final 55 conformer ensemble of the Vpr1-79–L-hHR23A223–363 complex.

The X-ray structure of Vpr in complex with the UBA2 domain revealed atomic details of Vpr’s Zn2+ -binding motif, which had not been resolved previously in either the X-ray structure of the DDB1/DCAF1/Vpr/UNG2 complex23 (PDB: 5JK7) or in the NMR structure of free Vpr36. The Zn2+ atom is coordinated by residues His33, His71, Cys76 and His78 at the C-terminal end of Vpr’s helix α3 (Fig. 1d and Supplementary Fig. 1f). His78 is the penultimate amino acid in the current Vpr construct and the Zn-binding cluster is important for maintaining the global and local architecture of Vpr.

Vpr/UBA2 domain binding interface

The binding interfaces on both Vpr and the UBA2 domain are extensive, burying a surface area of 760 Å2. Amino acids F34, W38, L42, I46, I63, L67 and I70 (helices α2 and α3) of Vpr pack against UBA2 residues F354, L350, A343, A340, Q339 and L336 (helices α3 and α2) in an antiparallel manner (Fig. 2a–c and Supplementary Fig. 3a–c). In addition, residues at the C- and N-terminal ends of Vpr’s α2 and α3 helices, H45, Y50, R73 and H71 and UBA2′s α2 and α3 helices, Q339, E345, N347 and N359, engage in hydrogen bonding and ionic interactions that contribute to binding (Fig. 2a). Amino acids in the Vpr–UBA2 intermolecular interface are listed in Supplementary Table 1. Within the UBA2 domain, an intramolecular NH···pi H-bond between the Q358 Nε2H2 amino group and the F354 aromatic ring is seen in the structure (Fig. 2a, b, orange dashed line), evidenced by the presence of intramolecular NOEs (Supplementary Fig. 3e) and a very large ring current-induced upfield shift of the Q358 amino group hydrogens (6.30, 6.46 ppm). The F354 aromatic ring is inserted into a small hydrophobic pocket lined by the side chains of Vpr’s F34, W38 and L67 (Fig. 2a–c). These binding interfaces in the Vpr1–79–L-hHR23A223–363 complex are clearly delineated by intermolecular NOEs (Fig. 2d, e, right and Supplementary Fig. 3e, f). The 3D NOESY data of the Vpr1–79–L-hHR23A223–363 complex were cross-checked and confirmed by comparison with the 3D NOESY data recorded on a sample of free hHR23A223–363 (Fig. 2e, left). As illustrated, intermolecular NOEs between the A340 HN of hHR23A and L67 Hδ of Vpr are only present in the spectrum of the Vpr1–79–L-hHR23A223–363 complex (Fig. 2e, right) and not in that of the free hHR23A223–363 (shown by two X symbols in Fig. 2e, left).

Fig. 2: The Vpr and UBA2 interface in the Vpr1-79–L-hHR23A223–363 complex.
figure 2

a Interactions between the α2 and α3 helices of Vpr1–79 and the α2 and α3 helices in the UBA2 domain in two orientations, b stereoview with Vpr1–79 at the front. The Vpr α1 and UBA2 α1 helices that are not involved in the Vpr/UBA2 interface are omitted for clarity. In a and b Vpr and UBA2 are shown in red and olive ribbon representation, respectively, with selected sidechains in ball-and-stick representation. c worm (Vpr) and surface (UBA2) representations, with the Vpr α1 helix and residues 51–62 omitted for clarity. Vpr is shown in red and UBA2 in olive. Selected H-bonds and the Zn-coordinating bonds are indicated by dotted black lines and the NH2–pi H-bond in orange. Interacting residues in UBA2 are labeled and colored in gold (hydrophobic), red (negatively charged) and blue (positively charged). d Selected regions of the 3D 13C-edited NOESY spectrum of the Vpr1–79–L-hHR23A223–363 complex, illustrating NOEs involving Vpr L67 Hδ methyl protons. e Selected regions of the 3D 15N-edited NOESY spectra of free hHR23A223–363 (left) and the Vpr1–79–L-hHR23A223–363 complex (right), illustrating intra-hHR23A223–363 NOEs and inter-molecular NOEs between hHR23A223–363 and Vpr1–79 for the A340 HN proton. The missing intermolecular cross-peaks in the free hHR23A223–363 spectrum are indicated by the symbols X. f GST-pull down data for WT and mutant hHR23AFL proteins (top) and VprFL (bottom). Source data are provided as a Source Data file. Three independent experiments were performed yielding similar results.

The Vpr/UBA2 interface was validated by mutagenesis of the involved amino acids on both hHR23AFL and VprFL. Several mutants of hHR23AFL were examined: the E345A/N347A mutant was tested, since E345 and N347 form a salt bridge and a hydrogen bond with Vpr Y50 and H45, respectively (Supplementary Fig. 3a); the F354A/Q358A mutant was assessed since the aromatic side chain is integral to the UBA2 structure and thus it was expected that this mutation would compromise the integrity of the fold (Fig. 2a–c and Supplementary Fig. 3b); the single Q339A mutant was tested, since Q339 forms a salt bridge with R73 of Vpr at the periphery of the Vpr–UBA2 interface (Fig. 2a–c and Supplementary Fig. 3c). The two double mutants, E345A/N347A and F354A/Q358A, abolished Vpr binding (Fig. 2f, top panel), while the single mutant Q339A had little effect. On the Vpr side, H45A, Y50S, R73A and the double mutant L67A/I70A were tested. All of these VprFL mutants exhibited impaired binding to hHR23AFL (Fig. 2f, bottom panel). Thus, all these residues are implicated in structural integrity or binding and changing them either destabilizes Vpr’s fold or removes important contacts, thereby indirectly or directly interfering with binding.

Previous work in our37 and other25 laboratories showed that hHR23A’s UBA2 domain, but not the UBA1 domain, is involved in Vpr binding. Indeed, the structure presented here and our site-directed mutagenesis experiments validate this model. Most of the UBA2 residues that are important for the interaction with Vpr (Fig. 2a–c) are not present in the UBA1 domain, such as L336 (R179 in UBA1), L350 (R193 in UBA1), F354 (Y197 in UBA1), Q339 (A182 in UBA1), E345 (Y188 in UBA1), Q358 (G201 in UBA1) and N359 (I202 in UBA1) (Supplementary Fig. 3d).

Vpr/XPCB domain binding interface

The XPCB domain interacts with the α1 and α3 face of Vpr (Fig. 1b, c), burying a surface area of 670 Å2, somewhat smaller than the UBA2–Vpr interface. The binding interface between Vpr and the XPCB domain (Fig. 3a–d) in the NMR structure of Vpr1–79–L-hHR23A223–363 was identified by several intermolecular NOEs in the 3D NOESY spectrum (Fig. 3e, f, right and Supplementary Fig. 3g), cross-checked against NOEs present in the 3D NOESY data of free hHR23A223–363 (Fig. 3e, f, left) where the intermolecular NOEs between the hHR23A I281Hδ1/L284Hδ2 and the Vpr F72/F69 aromatic resonances are missing. The main contacts involve the phenyl rings of F69 and F72 of Vpr that insert into a hydrophobic cleft on XPCB (Fig. 3a–d). In the current structure, Vpr F69 is surrounded by a cluster of XPCB hydrophobic residues that include I248, L255, L259, L270, I273, F280, I281 and L284. The Vpr F72 side chain is bordered by XPCB I248, P252, L255, F280, I281 and L284. In addition, an intermolecular NH···pi interaction is present between a side-chain Nε2H2 proton of XPCB’s Q277 and the phenyl ring of Vpr’s F69 (Fig. 3a, c, orange dashed line), consistent with the observation of the intermolecular NOEs (Supplementary Fig. 3g) and the large ring current-induced upfield shift of one of the Q277 Nε2H2 protons from the free positions of 7.36 and 6.55 ppm to 7.01 and 6.19 ppm. Several other H-bonds between Vpr and the XPCB domain are also observed: E29–E278, Q66–R275 and Q66–S274 (Fig. 3a–d). All residues directly involved in the Vpr–XPCB intermolecular interface, as evidenced by inter-molecular NOEs, are summarized in Supplementary Table 2. Notably, F69 is a key Vpr residue for DCAF1 binding23, suggesting that DCAF1 and XPCB binding is mutually exclusive (see also below).

Fig. 3: The Vpr and XPCB interface in the Vpr1–79–L-hHR23A223–363 complex.
figure 3

a, b Interactions between Vpr1–79 and the XPCB domain in the Vpr1–79–L-hHR23A223–363 complex, highlighting residues F69 and F72 in Vpr. c stereoview in a different orientation. In ac Vpr and XPCB are shown in red and teal ribbon representation, respectively, with selected sidechains in ball-and-stick representation. d worm (Vpr) and surface (XPCB) representations. Vpr is shown in red and the XPCB domain in teal. Selected amino acids are shown in ball-and-stick representation (Vpr), and H-bonds and the Zn-coordinating bonds are indicated by dotted black lines and the NH2–pi H-bond in orange. Interacting residues in the XPCB domain are labeled and colored in gold (hydrophobic), red (negatively charged) and blue (positively charged). e, f Selected regions of the 3D 13C-edited NOESY spectra of free hHR23A223–363 (left) and the Vpr1–79–L-hHR23A223–363 complex (right), illustrating intra-hHR23A223–363 NOEs and inter-molecular NOEs between hHR23A223–363 and Vpr1–79 for I281 Hδ (e) and L284 Hδ (f) methyl protons.

HIV-1 Vpr binds both the hHR23A and hHR23B isoforms

Most studies concerning NER have focused on hHR23B, rather than hHR23A. Given that the XPCB and UBA2 domains of hHR23A and hHR23B exhibit very high amino acid sequence identity (89%), we hypothesized that Vpr also interacts with hHR23B and investigated this possibility using in vitro and in vivo assays. Indeed, GST pull-down experiments indicated an interaction between Vpr and hHR23B, with the binding region localized to the C-terminal half of hHR23B (Fig. 4a). In addition, immunoprecipitation experiments revealed the presence of Vpr in both hHR23A (Fig. 4b, top) and hHR23B (Fig. 4b, bottom) immune complexes isolated from HEK293T cells co-expressing either hHR23 isoform together with Vpr, confirming that Vpr can form a complex with either hHR23 isoform in vivo. We also tested the effect of selected Vpr amino acid changes for binding hHR23A and hHR23B. Significantly, none of the variants tested, including the D52R/W54R one in which key interactions with the DNA repair protein UNG2 were abrogated, or the Vpr R80A variant, which no longer is able to activate G2 DNA damage checkpoint44, abolished binding to either hHR23 isoform, consistent with the results of our structural analyses of Vpr–hHR23A complex. In contrast, the Vpr F69A substitution, which disrupts Vpr binding to DCAF123, resulted in a reduced Vpr protein steady state level in cell extracts and binding to hHR23 (Fig. 4b). To ascertain whether the latter was simply due to the lower level of Vpr F69A protein in cells, or whether the F69 side chain is indeed critical for hHR23 binding, dose response binding experiments were performed. They were conducted under conditions where Vpr WT and the F69A variant were present at similar levels. Interestingly, similar amounts co-precipitated with hHR23A (Fig. 4c). This suggests that F69 is not critical for hHR23 binding, and that contacts made by other residues, including Q66 and F72, with the XPCB domain, as well as contacts by W38, L67 and I70 with the UBA2 domain are sufficient to stabilize the complex.

Fig. 4: Vpr binds both the hHR23A and hHR23B isoforms and the hHR23/XPC DNA recognition complex.
figure 4

a Recombinant GST-hHR23A, residues 1–363, 1–217 and 218–363 and GST-hHR23B, residues 1–409, 1–261 and 262–409 were used in GST-pull down experiments with recombinant VprFL. Three replicates were performed for each experiment yielding similar results. b Vpr mutant binding to hHR23A (top) and hHR23B (bottom). FLAG-tagged hHR23AFL (top) or hHR23BFL (bottom) complexes were immunoprecipitated, via FLAG-tag, from HEK293T cell extracts transiently co-expressing the respective hHR23 isoform and HA-tagged VprFL wild type (WT) or with the following mutations: D52R/W54R (52/54), F69A (69), R80A (80). Cell extracts and hHR23 immune complexes were analyzed by immunoblotting with antibodies specific for the FLAG and HA epitope tags, to detect the ectopically expressed FLAG-hHR23A, FLAG-hHR23B and HA-Vpr proteins. c Vpr F69A mutant binding to hHR23A. Expression levels of the HA-Vpr (F69A) and Vpr WT were varied by transfecting different amounts of plasmids expressing these proteins and their binding to FLAG-hHR23A was assessed as described above. d Vpr binds the hHR23/XPC complex. FLAG-tagged XPC, HA-tagged hHR23AFL and myc-tagged VprFL were expressed alone or in combinations in HEK293 T cells. Cell extracts and XPC immune complexes were analyzed by immunoblotting as described above except that the endogenously expressed hHR23B(en) protein was detected with an antibody specific for the hHR23B isoform. Immune complexes were revealed with fluorescent secondary antibodies and fluorescent signals quantified using a Li-Cor Odyssey imager. Relative levels of the indicated proteins in cell extracts and immune-complexes are shown. bd Two biological replicates were performed for each experiment yielding similar results. The biological replicate of the experiment shown in panel d is shown in Supplementary Fig. 4. ad Source data are provided as a Source Data file.

HIV-1 Vpr binds the hHR23A/XPC complex

hHR23 binds XPC, and this complex functions in DNA repair. Independently, hHR23 is also involved in ubiquitin-mediated proteolytic pathways. The latter does not require its association with XPC. To assess whether hHR23 has the potential to connect Vpr to NER, we asked whether Vpr can bind hHR23 in complex with XPC. To this end, XPC was co-expressed with Vpr in the absence or presence of increasing amounts of hHR23A, and XPC immune complexes were analyzed by immunoblotting for XPC, ectopically expressed hHR23A, endogenously expressed hHR23B isoform, and Vpr.

As shown in Fig. 4d and Supplementary Fig. 4, ectopic hHR23A expression resulted in increased amounts of Vpr co-precipitating with XPC. The low baseline levels of Vpr found in XPC immune complexes in the absence of ectopically expressed hHR23A probably reflect Vpr recruitment into the XPC complex by endogenously expressed hHR23A(B) isoforms, since the endogenous hHR23B is readily detectable in the XPC immune complexes. These findings support the model in which hHR23A(B) connects Vpr to hHR23A(B)/XPC mediated functions but does not exclude its involvement with XPC-independent hHR23 functions.

Vpr interactions with hHR23A and DCAF1 are mutually exclusive

Previous genetic and biochemical studies linked several cellular effects of Vpr to its binding to DCAF1 and, by extension, to the subversion of CRL4DCAF1 E3. Interestingly, comparison between the Vpr1–79–L-hHR23A223–363 (this study) and the UNG2/Vpr1–79/DCAF11045–1396/DDB1 (PDB: 5JK7) structures reveals that both DCAF1 and hHR23A interact with the α3 helix of Vpr (Fig. 5l–n and Supplementary Fig. 5a, b). However, engaging these different binding partners results in only a small change in the Vpr structure, namely a slight shift in the Vpr α3 helix (Supplementary Fig. 1g). The buried surface area in the Vpr1–79/DCAF11045–1396 region is slightly larger than in the Vpr1–79–L-hHR23A223–363 complex (1670 vs. 1430 Å2, respectively) and removal of DCAF1 residues 1045–1056 permitted purification of DCAF1 without DDB115, consistent with previous findings about DDB1 binding23. Therefore, our data suggest that Vpr interactions with hHR23A and DCAF1 are competitive, with binding to DCAF1 likely of higher affinity. We hence tested whether DCAF11057–1396 can compete and displace hHR23A from Vpr1–79 (Fig. 5a–e). When using DCAF11057–1396 in the presence of a 1:1 (M/M) mixture of Vpr1–79 and hHR23AFL in size exclusion column chromatography, an elution peak for free hHR23AFL (Fig. 5d, peak 4; Fig. 5e, lane 4) and a peak for the Vpr1–79/DCAF11057–1396 complex (Fig. 5d, peak 5; Fig. 5e, lane 5) is observed, confirming that Vpr binds DCAF11057–1396 more tightly than hHR23AFL. This again confirms that both these Vpr-binding proteins engage the same surface on Vpr for their interaction, namely helix α3, as structurally confirmed by the best-fit superposition of the Vpr1–79–L-hHR23A223–363 and the Vpr1–79/DCAF11045–1396 complex structures (Fig. 5l–n and Supplementary Fig. 5a, b). Both hHR23A223–363 and DCAF11045–1396 utilize hydrophobic residues to contact F69, I70 and F72 and charged residues to form hydrogen bonds with Q65 and Q66 (Fig. 5n). Non-shared interactions include residues from helices α2 and α3 (N41, I74 and R77), which bind the UBA2 domain, and the Vpr N-terminus that wraps around the side of DCAF1’s WD40 motif (Fig. 5n). The higher Vpr-binding affinity of DCAF1 was further confirmed by isothermal titration calorimetry (ITC): DCAF1 exhibited a Kd of 116 ± 47 nM while the hHR23A Kd was 640 ± 408 nM (Supplementary Fig. 5c, d). The above findings clearly demonstrate that Vpr binding to DCAF1 and hHR23 is mutually exclusive and suggest that hHR23 provides an alternative to the DCAF1 (CRL4DCAF1 E3)-mediated mechanisms for Vpr to exert its function.

Fig. 5: hHR23A is displaced from the Vpr/hHR23A complex by DCAF1.
figure 5

Analytical gel-filtration chromatography elution profiles of a DCAF11057–1396, b hHR23AFL, c the Vpr1–79/hHR23AFL complex, d an equimolar mixture of the Vpr1–79/hHR23AFL complex and DCAF11057–1396 and e SDS–PAGE analysis of the elution peaks; Lane numbers correspond to peak numbers in (ad). Analytical gel-filtration chromatography elution profiles of f hHR23AFL, g the VprFL/hHR23AFL complex, h UNG2, i an equimolar mixture of hHR23AFL and UNG2, j an equimolar mixture of the VprFL/hHR23AFL complex and UNG2 and k SDS–PAGE analysis of the elution peaks. Lane numbers correspond to peak numbers in (fj). Two independent experiments were performed yielding similar results. l, m Molecular model of the UNG2/Vpr1–79/hHR23A223–363 complex based on the superposition of the UNG2/Vpr1–79/DCAF11045–1396 complex and the current Vpr1–79–L-hHR23A223–363 complex without (l) and with displaying DCAF1 (m). n Surface representation of Vpr1–79 in two views, highlighting residues with DCAF11045–1396 contacts (blue), with both DCAF11045–1396 and hHR23A223–363 contacts (silver), and with hHR23A223–363 UBA2 only contacts (olive). Vpr1–79 residues that do not interact with DCAF11045–1396 or hHR23A223–363 are shown in red.

The hHR23A223–363 interaction with Vpr1–79 spans two of the three surfaces created by Vpr’s three helix bundle (Fig. 1b, c), permitting the third surface, particularly Vpr helices α1 and α2, to potentially engage in additional interactions. Previous work by our laboratory demonstrated that UNG2 binds to this third surface (PDB: 5JK7)23. We, therefore, tested whether the binary Vpr/hHR23A complex can interact with UNG2. Biochemical experiments, including size exclusion column chromatography, revealed that a ternary UNG2/Vpr/hHR23A complex can indeed be formed (Fig. 5f–k). The UNG2 association with hHR23 is strictly Vpr dependent, since, in the absence of Vpr, the mixture of hHR23A and UNG2 elutes as two individual peaks (Fig. 5i, k). A structural model for such a complex is shown in Fig. 5l. Whether the UNG2/Vpr/hHR23A complex forms in HIV-1-infected cells and plays a biological role remains to be determined.


HIV-1 Vpr, a small adaptor protein, is the prototype of a large family of primate lentiviral accessory virulence factors. It has been extensively studied over recent years, yet its mode of interaction with target proteins remains poorly understood. The structure of the HIV-1 Vpr/hHR23A complex reported here sheds new light on the molecular mechanisms that underlie the remarkable versatility of this adapter protein and its ability to engage in specific interactions with a large number of structurally unrelated protein partners. Our findings also provide new insights into the cellular mechanisms that Vpr is capable of targeting.

The core three helix bundle structure of Vpr provides different sides/interfaces to engage interacting proteins, while the unstructured N- and C-terminal regions potentially provide additional interaction motifs. Previous studies of HIV-1 Vpr complexes with DCAF1 and post-replication DNA repair proteins targeted by Vpr revealed two distinct Vpr interfaces: a DCAF1-binding interface and a substrate recruitment interface20,23 — the latter interacting with multiple targets including UNG2, MUS81 and HLTF17,20,23. Strikingly, the current structure of the Vpr/hHR23A complex reveals that hHR23 binds a surface on Vpr that largely overlaps with that engaged by DCAF1. This is remarkable, since hHR23A223–363 and DCAF11045–1396 exhibit entirely different three-dimensional structures (Fig. 5m and Supplementary Fig. 5a, b). The ability of Vpr to use the same surface to interact with two structurally unrelated partners, such as hHR23A and DCAF1, is unusual, possibly indicating that other proteins may potentially be engaged.

Both hHR23A and DCAF1 bind the solvent exposed sides of Vpr’s α3 helix, with only a few contacts that are specific to either UBA2 of hHR23A, such as N41 located in the α2 helix and I74 and R77 in the α3 helix, or to DCAF1, such as Vpr’s N-terminal region (Fig. 5n). These divergent, but important contacts probably contribute to the slightly higher affinity of DCAF11045–1396 for Vpr, compared to that of hHR23AFL. A similar strategy is evident in Vpr’s recruitment of substrates: Vpr utilizes both common and distinctive interfaces to recruit different post-replication DNA repair proteins to the CRL4DCAF1 E3 ligase for ubiquitin-dependent proteasomal degradation17,20,23. This structural adaptation may also be important in the specific recruitment of a multitude of additional substrate proteins tentatively identified by recent proteomic studies45. Together, these findings illustrate the remarkable versatility of HIV-1Vpr and reveal how it can interact specifically with a wide array of structurally dissimilar cellular targets.

Key residues involved in hHR23A binding are conserved in all HIV-1 Vpr isolates, yet absent in HIV-2 Vpr isolates (Sequences in the Los Alamos Data base; Supplementary Fig. 5e). This suggests that HIV-1 Vpr is uniquely tailored to engage hHR23A, consistent with a previous report46. It also contrasts with the observation that the DCAF1 interface residues are entirely conserved among HIV-1 and HIV-2 Vpr proteins. Since hijacking of CRL4DCAF1 E3 does not fully account for all known cellular effects of HIV-1 Vpr25,26,46,47, its interactions with hHR23A(B) are likely biologically relevant. Considering that Vpr can interact with both hHR23A and hHR23B, and both proteins appear to have largely overlapping functions, binding of Vpr may impact downstream pathways associated with both isoforms.

It is evident from our biochemical studies that Vpr associates with the XPC/hHR23A complex that plays key roles in post-replication DNA repair as well as transcription. Since Vpr binding to hHR23 does not require the presence of XPC in the complex, hHR23 probably acts as a bridge, linking Vpr with XPC. Although the atomic structure of the hHR23A/XPC complex, and in particular the detailed interaction between the XPCB domain of hHR23A and XPC, has not been elucidated yet, this possibility is consistent with predictions from the crystal structure of the homologous yeast Rad23/Rad4 complex48. In this structure, the UBA2 domain of Rad23 makes only a few contacts with Rad4, leaving most of the UBA2 domain available for interactions with other proteins, e.g, Vpr (Supplementary Figs. 6 and 7). Although the relative orientation between the domains varies considerably, given the long flexible linker between them, it is nonetheless possible to achieve a satisfactory superposition without steric clashes by rotating the XPCB domain (Supplementary Figs. 6 and 7). Thus, both the homologous human and yeast complexes appear to share similar overall architecture that is predicted to allow for Vpr binding to hHR23A in the presence of XPC.

Although the exact role of the Vpr interaction with the hHR23/XPC complex in HIV-1 biology is not yet known, possible scenarios are suggested by known functions of hHR23/XPC complexes and those of HIV-1 Vpr. In particular, the hHR23/XPC complex recognizes lesions that thermodynamically destabilize DNA duplexes in the early steps of post-replication nucleotide excision repair, including lesions resulting from oxidative DNA damage29. HIV-1 reverse transcriptase is error prone, and Vpr has been shown to modulate HIV-1 mutation rates as well as other functional aspects of cellular post-replication DNA repair machinery49,50. The latter includes antagonizing select enzymes that mediate processing of branched DNA structures as well as BER4,15,17,21,51. This antagonism, mediated by Vpr interacting with the CRL4DCAF1 E3 ligase, relieves restrictions imposed by these enzymes on HIV-1 replication4,22,52. In contrast, Vpr’s interaction with hHR23/XPC does not involve DCAF1 and does not lead to its ubiquitination and degradation. Thus, it may well be that Vpr recruits the hHR23/XPC complex to facilitate some aspect(s) of proviral DNA repair. Intriguingly, our tentative conclusion that the interaction with hHR23 is specific to HIV-1 Vpr fits well with the previous finding of a more robust remodeling of the cellular DNA repair machinery by HIV-1, compared to HIV-2 Vpr19. This is also consistent with the model in which Vpr aids HIV-1 replication by modulating the DNA repair function of the hHR23/XPC complex. Of note, whereas HIV-1 Vpr is known to activate DNA damage checkpoint leading to cell cycle arrest in G2 phase, the Vpr–hHR23 interaction is not required for this function (Supplementary Figs. 8 and  9) consistent with previous reports25,26,46.

HIV-1 Vpr activates expression of HIV-1 both prior to integration and post integration. It, therefore, may also modulate expression of cellular genes53,54,55. Interestingly, hHR23/XPC is involved in transcriptional co-activation via histone acetylation and chromatin activation at a subset of RNA polymerase II promoters31,32, thus suggesting another role for the Vpr/hHR23 interaction. It is conceivable that the recruitment of the hHR23/XPC complex to active chromatin plays a role in transcriptional effects exerted by HIV-1 Vpr. Future studies are needed to address any possible involvement of the hHR23/XPC complex in the modulation of gene expression and cellular DNA repair processes by HIV-1 Vpr.

In summary, this study and previous structural and biochemical work suggest varied ways by which HIV-1 Vpr can coordinate specific interactions with a large number of structurally unrelated proteins. All lentiviral Vpr family proteins share their core structure with HIV-1 Vpr, yet exhibit extensive amino acid sequence variation on their surfaces. This sequence variability in conjunction with the molecular organization into a plastic three-helix bundle permits promiscuity in protein–protein interactions, providing a versatile means to interfere with multiple key cellular pathways.


Cloning and construction of plasmids

DNA coding for hHR23A223–363 and Vpr1–79–L-hHR23A223–363 was inserted into pET21 (EMD Chemicals). hHR23A223–363 and NusA-Vpr1–79 gene constructs were inserted into a pET-DUET vector (EMD Chemicals) for co-expression. The Vpr1–79–L-hHR23A223–363 construct contained a His6 tag (LEHHHHHH) at its C-terminus. For mammalian cell expression, cDNAs encoding epitope-tagged full-length (FL) hHR23A, hHR23B, XPC and HIV-1 NL4-3 Vpr wild type and mutant alleles were cloned into pCG vector56. All primers used are listed in Supplementary Table 3.

Protein expression and purification

hHR23A223–363, hHR23AFL, Trx-hHR23AFL, NusA–Vpr1–79, UNG2, Vpr1–79–L-hHR23A223–363, hHR23A223–363–L-Vpr1–79, Vpr1–79/hHR23A223–363, Vpr1–79/hHR23AFL and VprFL/hHR23AFL complexes were expressed or co-expressed in E. coli Rosetta 2 (DE3) or Rosetta 2 (DE3) pLysS cells (EMD Chemicals) in the presence of 0.1 mM ZnCl2, using 0.5 mM isopropyl 1-thio-β-d-galactopyranoside for induction over 16 h at 18 °C. Uniform 2H-, 15N-, and 13C- labeling of the proteins was achieved using modified minimal medium, containing 2H2O, 15NH4Cl and 13C6-glucose as deuterium, nitrogen and carbon sources, while unlabeled proteins were prepared in Luria-Bertani (LB) medium. Cells were harvested by centrifugation at 6000×g and re-suspended in lysis buffer, containing 50 mM sodium phosphate (pH 7.5), 10 mM imidazole and 200 mM NaCl. Cells were lysed using a microfluidizer (Microfluidics, MA) or, for hHR23A223–363 and Vpr1–79–L-hHR23A223–363 samples, by sonication (Sonicator 3000; Misonix, Farmingdale, NY). The lysed cells were centrifuged at 40,000×g, and the supernatant was applied to a 5-ml HisTrap (GE healthcare) His6-tag affinity column, equilibrated in lysis buffer. Bound proteins were eluted using a linear gradient of 0–0.5 M imidazole. Protein containing fractions were further purified over a HiPrep Superdex 75 or HiPrep Superdex 200 (2.6 cm × 60 cm, GE healthcare) gel filtration column, equilibrated in 25 mM sodium phosphate buffer (pH 7.5), 100 mM NaCl, 2 mM DTT and 0.02% sodium azide. Vpr1–79–L-hHR23A223–363, Vpr1–79/hHR23A223–363, Vpr1–79/hHR23AFL and VprFL/hHR23AFL complexes were further purified over a HiTrap Q column, equilibrated in 25 mM sodium phosphate buffer (pH 7.5), 2 mM DTT and 0.02% sodium azide. Bound proteins were eluted using a linear gradient of 0–1 M NaCl. Protein fractions were pooled, concentrated for crystallization or buffer-exchanged in Amicon Ultra concentrators (Millipore) for NMR studies against NMR buffers: 10 mM MES (pH 6.5), 10 mM HEPES (pH 7.2 or 7.5) or 25 mM sodium phosphate (pH 7.2 or 7.5), each containing 50 mM NaCl, 2–5 mM DTT or 1 mM TCEP, 0.1 mM ZnSO4, 0.1 mM EDTA and 0.02% sodium azide. The correct molecular masses of proteins were confirmed by LC–ESI–TOF mass spectrometry (Bruker Daltonics, Billerica, MA). DCAF1157–1396 was expressed in sf9 insect cells and was purified using a 5 ml HisTrap (GE healthcare) and HiPrep Superdex200 (2.6 cm × 60 cm, GE healthcare) gel filtration column as described above.

Crystallization, structure determination and refinement

Crystals for Vpr1–79–L-hHR23A223–363 were grown at 16 °C by hanging drop vapor diffusion, using a mixture of 0.5 μl protein (25 mg/ml) with 0.5 μl crystallization buffer (0.1 M Tris, pH 7.5, 10% PEG 4000). Initial crystals were improved by streak seeding. Crystals were cryoprotected by the addition of 35% (v/v) glycerol. Crystals belonging to space group P3121, diffracting to 2.2 Å, were used for structure determination. Data were collected at the 9-2 SSRL (wavelength 0.979 Å, temperature 100 K). Diffraction data were processed, integrated and scaled using XDS57. The structure was solved by de novo Zn2+ single-wavelength anomalous dispersion (Zn-SAD) phasing. Models were refined using the program BUSTER41, followed by several cycles of manual model building and B factor sharpening in Coot43. Crystallographic statistics are summarized in Table 1.

NMR spectroscopy

NMR data were collected on Bruker 600, 700, 800, and 900 MHz AVANCE spectrometers. All spectrometers were equipped with z-axis gradient, triple resonance cryoprobes. Experiments were performed at 298 K. A homonuclear 2D NOESY spectrum was acquired on unlabeled Vpr1–79–L-hHR23A223–363. Heteronuclear 2D 1H–15N HSQC and 1H–13C HSQC and 3D HNCACB, HN(CO)CACB, HNCA, HN(CO)CA, HBHACONH, HCCCONH, CCCONH, HCCH-TOCSY (mixing time 10.9 ms) and simultaneously 13C/15N-edited NOESY (mixing time 100 ms) experiments were performed using either 15N- or 15N,13C-labeled Vpr1–79–L-hHR23A223–363, hHR23A223–363-L-Vpr1–79 (only 2D 1H–15N HSQC) and hHR23A223–363 samples. For 2H,15N,13C-labeled Vpr1–79–L-hHR23A223–363, 3D TROSY-type HNCACB, HN(CO)CACB spectra were also recorded. All data were processed with NMRPipe58 and TopSpin 3.1 (Bruker), and analyzed with CCPN59.

NMR structure determination

Structures were calculated in XPLOR-NIH60,61. An iterative approach with extensive manual cross-checking of all distance restraints against the NOESY data and the initial structures was employed using CCPN59. The final number of NOE-derived distance restraints is 7650, of which 338 are intermolecular (81 Vpr1–79/XPCB domain and 257 Vpr1–79/UBA2 domain), supplemented by 330 ϕ and ψ backbone torsion angles from TALOS62. In addition, a Zn2+ ion with tetrahedral coordination to the H33, H71, C76 and H78 side chains of Vpr1–79 was added at a late stage in the calculations, using distances from the X-ray structure of the Vpr1–79/UBA2 domain. In total, 960 structures were generated, and the 55 lowest energy structures were selected and analyzed using XPLOR-NIH63 and PROCHECK-NMR64. All structure figures except Supplementary Fig. 1e were generated with Pymol. Supplementary Fig. 1e was generated with MOLMOL65.

In-gel trypsin digestion for mass spectrometry

Gel bands containing (1) a Vpr1–79–L-hHR23A223–363 solution employed to set up crystallization trays (control) or (2) dissolved crystals of Vpr1–79–L-hHR23A223–363 were excised and proteins were digested with trypsin66. Briefly, gel bands were diced into small pieces (<1 mm3) and washed with a solution of 50% acetonitrile (ACN)/25 mM ammonium bicarbonate until no more visible stain was present. The gel pieces were then dehydrated with 100% ACN, reduced with 10 mM DTT at 56 °C for 1 h, followed by alkylation with 55 mM Iodoacetamide (IAA) at room temperature for 45 min in the dark. Excess DTT and IAA were removed by washing the gel pieces with 25 mM ammonium bicarbonate and then twice with 100% ACN. A solution containing 20 ng/µL sequencing grade modified trypsin (Promega Corporation, Madison, WI; catalog#V511A) and 25 mM ammonium bicarbonate was added to cover the gel pieces and digestion was carried out overnight at 37 °C. The resultant tryptic peptides were extracted from the gel with 70% ACN/5% formic acid (FA), vacuum dried, and reconstituted in 18 µL 0.1% FA for nanoflow liquid-chromatography tandem mass spectrometry (nLC–MS/MS analysis).

Tandem mass spectrometry

Tryptic peptides were analyzed by nLC–MS/MS using a NanoAcquity UPLC (Waters’ Corporation, Milford, MA) interfaced to a Velos Pro linear ion trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). For each analysis, a 1 µL volume of protein digest was injected onto a C18 column (PicoChip™column packed with Reprosil C18 3 μm 120 Å chromatography media in a 10.5 cm long, 75 μm ID column with a 15 μm tip, New Objective, Inc., Woburn, MA, USA) and eluted off to the mass spectrometer using a 37-min linear gradient of 3–35% ACN/0.1% FA at a flow rate of 300 nL/min. The Velos Pro was operated in positive ionization mode with a spray voltage of 1.95 kV and capillary temperature of 275 °C. Acquisition consisted of cycles of one full-scan MS1 (AGC of 3 × 104, 75 ms maximum ion accumulation time, and m/z range of 375–1800) followed by eight MS/MS spectra recorded sequentially for the most abundant ions in the ion trap (minimum signal required 1000 counts, 1 × 104 AGC target, 100 ms maximum injection time, isolation width 2m/z, normalized collision energy 35, and activation time 10 ms). Dynamic exclusion (30 s) was enabled to minimize redundant selection of peptides previously selected for MS/MS.

Mass spectrometry data analysis

Collected MS/MS spectra were searched using the MASCOT search engine v2.4.0 (Matrix Science Ltd., London, England)67 against the UniProt E. coli database, downloaded on September 5, 2014, with 5803 entries, plus the sequence for 23A2V, using the following modifications: static modification of cysteine carbamidomethylation (+57), variable modifications of methionine oxidation (+16), and protein N-terminal acetylation (+42). The mass tolerance was set to 1.4 Da for the precursor ions and 0.8 Da for the fragment ions. Peptide identifications were filtered using the PeptideProphetTM and ProteinProphet® algorithms with a protein threshold cutoff of 99%, minimum of 2 peptides, and peptide threshold cutofff of 90% implemented in ScaffoldTM v4.11.0 (Proteome software, Portland, OR).

GST pulldown assays

1000 pmol of GST-tagged WT hHR23A/B or mutant proteins were incubated with 1000 pmol NusA–VprFL in 500 μl PBS buffer (binding buffer) for 30 min at 4 °C. TEV cleavage (50 pmol) was carried out at 4 °C overnight. After centrifugation at 21,000×g for 20 min (to remove precipitated, excess Vpr) Glutathion-Sepharose 4B beads (50 μl of a 50% slurry) were added to the supernatant for 1 h at 4 °C. The beads were washed four times with 1 ml of binding buffer, followed by addition of 50 μl SDS–PAGE loading buffer. Proteins were separated by 4–20% SDS–PAGE, transferred to PVDF membrane, and detected by immunoblotting with an anti-His antibody (1:1000 dilution, cat. #05-949, clone His.H8, Millipore).

Transfections, immunoprecipitations and immunoblotting

Transfections of HEK 293T cells were performed using the calcium phosphate co-precipitation method68. Whole-cell extracts were immunoprecipitated with FLAG-M2 beads (Sigma-Aldrich). Immune complexes were eluted by competition with FLAG-peptide under native conditions, separated by SDS–PAGE and transferred to PVDF membranes for immunoblotting. Proteins were detected with antibodies specific for hHR23B (1:1000 dilution, cat. #13525, clone D4W7F, Cell Signaling Technology), FLAG- (1:1000, cat. #F1804, clone M2, Sigma), HA- (1:2000, 12CA5, PMID: 6204768) or myc- (1:1000, 9E10, PMID: 3915782) epitope tags, immune complexes revealed with fluorescent secondary antibodies specific for the mouse (1:10,000, IRDye 800CW Gt anti-mouse IgG Li-Cor cat. #926-322100, or rabbit IgG (IRDye 680RD Gt anti-rabbit IgG 1:10,000, Li-Cor cat. #926-68071) and fluorescent signals quantified using Li-Cor Odyssey imager and software version 3.0.30 (Li-Cor).

Cell cycle analysis

HEK293T cells were seeded in 12-well plates and co-transfected with plasmids expressing HIV-1 FLAG-tagged HIV-1 Vpr.wt or mutant proteins and GFP marker protein. 48 h post transfection, the cells were trypsinized, washed twice with PBS, fixed with 70% ethanol overnight, washed twice with PBS and stained with propidium iodide. GFP and propidium iodide fluorescence of the cells was analyzed by flow cytometry (BD LSRII). Data were analyzed using FlowJo software (Tree Star Inc., Ashland, OR). One-way ANOVA analysis was performed within GraphPad Prism 9.

Analytical gel filtration column chromatography

One hundred μl of proteins at 20 μM (hHR23AFL, VprFL/hHR23AFL complex, UNG2, an equimolar mixture of hH23AFL and UNG2 or VprFL/hHR23AFL complex and UNG2) or 60 μl of proteins at 21 μM (DCAF11057–1396, hHR23AFL, Vpr1–79/hHR23AFL complex or an equimolar mixture of Vpr1–79/hHR23AFL complex and DCAF11057–1396) was injected onto a 24-ml analytical Superdex75 column (equilibrated in 25 mM sodium phosphate, 150 mM NaCl, 5% glycerol, 1 mM DTT and 0.02% NaN3) at a flow rate of 0.5 ml/min. Each peak was concentrated and analyzed by SDS–PAGE with Coomassie Blue staining.

Isothermal titration calorimetry (ITC)

Experiments were performed using a MicroCal PEAQ-ITC calorimeter (Malvern). Proteins were dialyzed overnight against 20 mM sodium phosphate buffer, pH 7.4, 100 mM NaCl and concentrated in an Amicon concentrator (EMD Millipore). NusA–Vpr1–79 protein (8.5 µM) was placed in the sample cell and either Trx-hHR23AFL or DCAF11045–1396/DDB1 proteins (100 μM) were added in aliquots at 18 °C. Data analyses were performed with the PEAQ-ITC analysis software.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The coordinates of the crystal structure of the Vpr1-79/hHR23A-UBA2 complex have been deposited to the Protein Data Bank under accession number PDB 6XQI. The coordinates of the NMR structures of the Vpr1–79–L-hHR23A223–363 complex have been deposited to the Protein Data Bank under accession number PDB 6XQJ, and the NMR assignments of the complex have been deposited to the Biological Magnetic Resonance Bank ( under entry number 30769, which is available at Previously published PDB accession codes that have been used in this study are 5JK7 (crystal structure of the DDB1/DCAF1/Vpr/UNG2 complex), 2QSF (crystal structure of the yeast Rad23/Rad4 complex), and 4UN2 (crystal structure of the UBA domain of Dsk2 in complex with ubiquitin). Source data are provided with this manuscript.


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The authors thank Teresa Brosenitsch for critical reading of the manuscript, Doug Bevan for computer, Michael J. Delk for NMR, Doowon Lee for in-house X-ray technical support, Cynthia Hale for setting up quantitative Western blotting, and Dewayne Falkner and Ailing Liu for assistance with the cell cycle assay experiments. We also thank Michael Becker and Craig Ogata at GM/CA (Argonne National Laboratory) for user support and Aina Cohen, Irimpan Mathews, Clyde Smith, and Ana Gonzalez at Stanford Synchrotron Radiation Lightsource (SSRL) for assistance during data collection. The ITC instrument was funded by NIH grant 1S10OD023481 (to A.M.G.). The tandem mass spectrometry analysis was supported by the UPMC Hillman Cancer Center Proteomics Facility, supported in part by award P30CA047904. This work was supported by NIH grant P50AI150481 (to A.M.G.).

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A.M.G. conceived and directed this study. C.H.B., J.J., M.D. and Y.W. prepared proteins. I.L.B., J.J. and C.H.B. conducted NMR experiments. I.L.B. analyzed NMR data and was responsible for the completion of the NMR/NOE assignments and the NMR structure determination of the Vpr1–79–L-hHR23A223–363 complex. Y.W. carried out crystallization experiments. S.W. carried out X-ray data collection and processing. G.C. performed structure refinement of the X-ray structure. G.C. and I.L.B. analyzed the NMR and X-ray structures and led in providing structural insight. G.C. and J.A. designed and supervised the biochemical experiments. Y.W. carried out mutagenesis and GST-pull down experiments. M.D. and Y.W. performed analytical gel-filtration column chromatography. X.Z. carried out ITC experiments. J.S. designed and supervised Vpr–XPC interaction studies, and C.H. carried out transfections, immunoprecipitations and immunoblotting. I.L.B. and G.C. prepared the initial draft of the manuscript and J.S. and A.M.G. led the final manuscript preparation. All authors reviewed, commented on, and approved the manuscript.

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Correspondence to Guillermo Calero or Angela M. Gronenborn.

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Byeon, IJ.L., Calero, G., Wu, Y. et al. Structure of HIV-1 Vpr in complex with the human nucleotide excision repair protein hHR23A. Nat Commun 12, 6864 (2021).

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