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Location of corneal epithelial stem cells

Abstract

Arising from: Majo, F., Rochat, A., Nicolas, M., Jaoude, G. A. & Barrandon, Y. Nature 456, 250–254 (2008).10.1038/nature07406; Majo et al. reply

The longstanding concept that corneal epithelial stem cells reside mainly in the limbus is supported by the absence of major corneal epithelial differentiation markers, that is, K3 and K12 keratins, in limbal basal cells (these markers are expressed, however, in corneal basal cells, thus distinguishing the mode of keratin expression in corneal epithelium from that of all other stratified epithelia), the centripetal migration of corneal epithelial cells, the exclusive location of slow-cycling cells in the limbal basal layer, the superior in vitro proliferative potential of limbal epithelial cells, and the transplanted limbal cells’ ability to reconstitute corneal epithelium in vivo (reviewed in refs 1–4). Moreover, previous data indicate that corneal and conjunctival epithelia represent two separate cell lineages (reviewed in refs 1–4). Majo et al.5 suggested, however, that corneal and conjunctival epithelia are equipotent, and that identical oligopotent stem cells are present throughout the corneal, limbal and conjunctival epithelia. We point out here that these suggestions are inconsistent with many known growth, differentiation and cell migration properties of the anterior ocular epithelia.

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References

  1. Schermer, A., Galvin, S. & Sun, T. T. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J. Cell Biol. 103, 49–62 (1986)

    CAS  Article  Google Scholar 

  2. Cotsarelis, G., Cheng, S. Z., Dong, G., Sun, T. T. & Lavker, R. M. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell 57, 201–209 (1989)

    CAS  Article  Google Scholar 

  3. Kenyon, K. R. & Tseng, S. C. Limbal autograft transplantation for ocular surface disorders. Ophthalmology 96 (5), 709–722; discussion 722–723 (1989)

    CAS  Article  Google Scholar 

  4. Lavker, R. M., Tseng, S. C. & Sun, T. T. Corneal epithelial stem cells at the limbus: looking at some old problems from a new angle. Exp. Eye Res. 78, 433–446 (2004)

    CAS  Article  Google Scholar 

  5. Majo, F., Rochat, A., Nicolas, M., Jaoude, G. A. & Barrandon, Y. Oligopotent stem cells are distributed throughout the mammalian ocular surface. Nature 456, 250–254 (2008)

    ADS  CAS  Article  Google Scholar 

  6. Sun, T.-T. & Green, H. Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature 269, 489–493 (1977)

    ADS  CAS  Article  Google Scholar 

  7. Wei, Z. G., Wu, R. L., Lavker, R. M. & Sun, T. T. In vitro growth and differentiation of rabbit bulbar, fornix, and palpebral conjunctival epithelia. Implications on conjunctival epithelial transdifferentiation and stem cells. Invest. Ophthalmol. Vis. Sci. 34, 1814–1828 (1993)

    CAS  PubMed  Google Scholar 

  8. Doran, T. I., Vidrich, A. & Sun, T.-T. Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22, 17–25 (1980)

    CAS  Article  Google Scholar 

  9. Wei, Z. G., Sun, T. T. & Lavker, R. M. Rabbit conjunctival and corneal epithelial cells belong to two separate lineages. Invest. Ophthalmol. Vis. Sci. 37, 523–533 (1996)

    CAS  PubMed  Google Scholar 

  10. Pellegrini, G. et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J. Cell Biol. 145, 769–782 (1999)

    CAS  Article  Google Scholar 

  11. Huang, A. J. & Tseng, S. C. Corneal epithelial wound healing in the absence of limbal epithelium. Invest. Ophthalmol. Vis. Sci. 32, 96–105 (1991)

    CAS  PubMed  Google Scholar 

  12. Auran, J. D. et al. Scanning slit confocal microscopic observation of cell morphology and movement within the normal human anterior cornea. Ophthalmology 102, 33–41 (1995)

    CAS  Article  Google Scholar 

  13. Collinson, J. M. et al. Clonal analysis of patterns of growth, stem cell activity, and cell movement during the development and maintenance of the murine corneal epithelium. Dev. Dyn. 224, 432–440 (2002)

    Article  Google Scholar 

  14. Nagasaki, T. & Zhao, J. Centripetal movement of corneal epithelial cells in the normal adult mouse. Invest. Ophthalmol. Vis. Sci. 44, 558–566 (2003)

    Article  Google Scholar 

  15. Nagasaki, T. & Zhao, J. Uniform distribution of epithelial stem cells in the bulbar conjunctiva. Invest. Ophthalmol. Vis. Sci. 46, 126–132 (2005)

    Article  Google Scholar 

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Sun, TT., Tseng, S. & Lavker, R. Location of corneal epithelial stem cells. Nature 463, E10–E11 (2010). https://doi.org/10.1038/nature08805

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